ZFIN ID: ZDB-FIG-180705-12
Liu et al., 2018 - Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply. Nature communications   9:1310 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 3

Hematopoietic defects in apoc2 and lpl mutants occur during HSPC expansion. a In situ hybridization with cmyb/runx1 and β-globin probes in WT, apoc2 and lpl mutants at 26 hpf, 30 hpf and 50 hpf. b In situ hybridization with cmyb/runx1, β-globin, rag1 (green arrows) and foxn1 (black arrows) probes in WT, apoc2 and lpl mutants at 80 hpf. foxn1 is a thymus development marker, used as a control. c, d Representative images and numbers of GFPlow cells (HSPCs, white arrows) in the CHT region of cd41:EGFP transgenic WT and apoc2 mutants at 54 and 80 hpf (n = 10 in WT and n = 8 in apoc2 mutant groups at 54 hpf; n = 6 in each group at 80 hpf). e, f Representative images and numbers of GFP-positive cells in the thymus region at 54 hpf (n = 9 in WT and n = 8 in apoc2 mutant groups) and at 4 dpf (n = 8 in each group). Scale bars, 200 μm in a and b; 50 μm in cf. Mean ± SEM; *P < 0.05 and ***P < 0.001 (Student’s t test)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP la2Tg control Long-pec caudal hematopoietic tissue hematopoietic multipotent progenitor cell IFL
Long-pec thymus primordium hematopoietic multipotent progenitor cell IFL
Protruding-mouth caudal hematopoietic tissue hematopoietic multipotent progenitor cell IFL
Day 4 thymus hematopoietic multipotent progenitor cell IFL
la2Tg ; apoc2sd38/sd38 control Long-pec caudal hematopoietic tissue hematopoietic multipotent progenitor cell IFL
Long-pec thymus primordium hematopoietic multipotent progenitor cell IFL
Protruding-mouth caudal hematopoietic tissue hematopoietic multipotent progenitor cell IFL
Day 4 thymus hematopoietic multipotent progenitor cell IFL
foxn1 AB control Protruding-mouth thymus ISH
apoc2sd38/sd38(AB) control Protruding-mouth thymus ISH
lplsd51/sd51(AB) control Protruding-mouth thymus ISH
hbbe1.1 AB control Prim-5 erythroblast ISH
Prim-15 erythroblast ISH
Long-pec erythroblast ISH
Protruding-mouth erythroblast ISH
apoc2sd38/sd38(AB) control Prim-5 erythroblast ISH
Prim-15 erythroblast ISH
Long-pec erythroblast ISH
Protruding-mouth erythroblast ISH
lplsd51/sd51(AB) control Prim-5 erythroblast ISH
Prim-15 erythroblast ISH
Long-pec erythroblast ISH
Protruding-mouth erythroblast ISH
myb AB control Prim-5 hematopoietic multipotent progenitor cell ISH
Prim-15 hematopoietic multipotent progenitor cell ISH
Long-pec hematopoietic multipotent progenitor cell ISH
Protruding-mouth hematopoietic multipotent progenitor cell ISH
apoc2sd38/sd38(AB) control Prim-5 hematopoietic multipotent progenitor cell ISH
Prim-15 hematopoietic multipotent progenitor cell ISH
Long-pec hematopoietic multipotent progenitor cell ISH
Protruding-mouth hematopoietic multipotent progenitor cell ISH
lplsd51/sd51(AB) control Prim-5 hematopoietic multipotent progenitor cell ISH
Prim-15 hematopoietic multipotent progenitor cell ISH
Long-pec hematopoietic multipotent progenitor cell ISH
Protruding-mouth hematopoietic multipotent progenitor cell ISH
rag1 AB control Protruding-mouth thymus ISH
Protruding-mouth thymus lymphocyte ISH
apoc2sd38/sd38(AB) control Protruding-mouth thymus ISH
Protruding-mouth thymus lymphocyte ISH
lplsd51/sd51(AB) control Protruding-mouth thymus ISH
Protruding-mouth thymus lymphocyte ISH
runx1 AB control Prim-5 hematopoietic multipotent progenitor cell ISH
Prim-15 hematopoietic multipotent progenitor cell ISH
Long-pec hematopoietic multipotent progenitor cell ISH
Protruding-mouth hematopoietic multipotent progenitor cell ISH
apoc2sd38/sd38(AB) control Prim-5 hematopoietic multipotent progenitor cell ISH
Prim-15 hematopoietic multipotent progenitor cell ISH
Long-pec hematopoietic multipotent progenitor cell ISH
Protruding-mouth hematopoietic multipotent progenitor cell ISH
lplsd51/sd51(AB) control Prim-5 hematopoietic multipotent progenitor cell ISH
Prim-15 hematopoietic multipotent progenitor cell ISH
Long-pec hematopoietic multipotent progenitor cell ISH
Protruding-mouth hematopoietic multipotent progenitor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apoc2sd38/sd38(AB) control Protruding-mouth erythroblast hbbe1.1 expression decreased amount, abnormal
Protruding-mouth hematopoietic multipotent progenitor cell myb expression decreased amount, abnormal
Protruding-mouth hematopoietic multipotent progenitor cell runx1 expression decreased amount, abnormal
Protruding-mouth thymus rag1 expression decreased amount, abnormal
Protruding-mouth thymus lymphocyte rag1 expression decreased amount, abnormal
la2Tg ; apoc2sd38/sd38 control Long-pec caudal hematopoietic tissue hematopoietic multipotent progenitor cell amount, normal
Long-pec thymus hematopoietic multipotent progenitor cell amount, normal
Protruding-mouth caudal hematopoietic tissue hematopoietic multipotent progenitor cell decreased amount, abnormal
Day 4 thymus hematopoietic multipotent progenitor cell decreased amount, abnormal
lplsd51/sd51(AB) control Protruding-mouth erythroblast hbbe1.1 expression decreased amount, abnormal
Protruding-mouth hematopoietic multipotent progenitor cell myb expression decreased amount, abnormal
Protruding-mouth hematopoietic multipotent progenitor cell runx1 expression decreased amount, abnormal
Protruding-mouth thymus rag1 expression decreased amount, abnormal
Protruding-mouth thymus lymphocyte rag1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.