|
Hematopoietic defects in apoc2 and lpl mutants occur during HSPC expansion. a In situ hybridization with cmyb/runx1 and ?-globin probes in WT, apoc2 and lpl mutants at 26?hpf, 30?hpf and 50?hpf. b In situ hybridization with cmyb/runx1, ?-globin, rag1 (green arrows) and foxn1 (black arrows) probes in WT, apoc2 and lpl mutants at 80?hpf. foxn1 is a thymus development marker, used as a control. c, d Representative images and numbers of GFPlow cells (HSPCs, white arrows) in the CHT region of cd41:EGFP transgenic WT and apoc2 mutants at 54 and 80?hpf (n?=?10 in WT and n?=?8 in apoc2 mutant groups at 54?hpf; n?=?6 in each group at 80?hpf). e, f Representative images and numbers of GFP-positive cells in the thymus region at 54?hpf (n?=?9 in WT and n?=?8 in apoc2 mutant groups) and at 4 dpf (n?=?8 in each group). Scale bars, 200??m in a and b; 50??m in c?f. Mean?±?SEM; *P?<?0.05 and ***P?<?0.001 (Student?s t test)
|
