FIGURE

Fig. 3

ID
ZDB-FIG-180705-12
Publication
Liu et al., 2018 - Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply
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Fig. 3

Hematopoietic defects in apoc2 and lpl mutants occur during HSPC expansion. a In situ hybridization with cmyb/runx1 and β-globin probes in WT, apoc2 and lpl mutants at 26 hpf, 30 hpf and 50 hpf. b In situ hybridization with cmyb/runx1, β-globin, rag1 (green arrows) and foxn1 (black arrows) probes in WT, apoc2 and lpl mutants at 80 hpf. foxn1 is a thymus development marker, used as a control. c, d Representative images and numbers of GFPlow cells (HSPCs, white arrows) in the CHT region of cd41:EGFP transgenic WT and apoc2 mutants at 54 and 80 hpf (n = 10 in WT and n = 8 in apoc2 mutant groups at 54 hpf; n = 6 in each group at 80 hpf). e, f Representative images and numbers of GFP-positive cells in the thymus region at 54 hpf (n = 9 in WT and n = 8 in apoc2 mutant groups) and at 4 dpf (n = 8 in each group). Scale bars, 200 μm in a and b; 50 μm in cf. Mean ± SEM; *P < 0.05 and ***P < 0.001 (Student’s t test)

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Long-pec to Day 4

Phenotype Detail
Acknowledgments
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