Fig. 1

Anemia in apoc2 mutant zebrafish. a Wright–Giemsa (Hema) and o-dianisine staining of peripheral blood cells from adult (18–20-month-old) male wild-type (WT) and apoc2 mutant zebrafish. b Quantitative results of peripheral blood cell count (n = 11 for WT and n = 9 for apoc2 mutant groups). c Representative bright field images and quantitative results of blood cell (yellow arrows) count in the caudal vein (outlined with white dashed lines) of WT and apoc2 mutants at 52 hpf (n = 8 in WT and n = 9 in apoc2 mutant groups) and 6.3 dpf (n = 11 for WT and n = 13 for apoc2 mutant groups). See also Supplementary Movies 1 and 2. d Wright–Giemsa staining of blood smears from WT and apoc2 mutants at 52 hpf and 6.3 dpf. Immature erythrocytes containing larger and less condensed nuclei are indicated with red arrows in a and d. Immature erythrocytes with weaker hemoglobin staining are indicated with green arrows in a. e o-Dianisine staining of 52 hpf and 6.3 dpf WT and apoc2 mutant embryos. f Effect of lomitapide: WT and apoc2 mutants were treated with 5 μM lomitapide starting from 2 dpf until embryos were fixed at 6 dpf for ORO staining. Black arrows point to intestinal lipid accumulation and green arrows to circulating lipids. g Blood cell counts in the caudal vein of WT, apoc2 mutants and apoc2 mutants treated with lomitapide at 6.3 dpf (n = 5 in each group). h Blood cell counts in the caudal vein of 14 dpf WT, apoc2 mutants fed with normal diet and apoc2 mutants fed with low-fat diet (LFD) starting at 5 dpf (n = 4 in WT and apoc2 mut groups each; n = 5 in apoc2 + LFD group). Scale bars, 20 μm in a, c, and d, 100 μm in e, and 200 μm in f. Mean ± SEM; ***P < 0.001 (Student’s t test)