ZFIN Image: Liu et al., 2018, Fig. 3

Image

Figure Caption

Fig. 3

Hematopoietic defects in apoc2 and lpl mutants occur during HSPC expansion. a In situ hybridization with cmyb/runx1 and β-globin probes in WT, apoc2 and lpl mutants at 26 hpf, 30 hpf and 50 hpf. b In situ hybridization with cmyb/runx1, β-globin, rag1 (green arrows) and foxn1 (black arrows) probes in WT, apoc2 and lpl mutants at 80 hpf. foxn1 is a thymus development marker, used as a control. c, d Representative images and numbers of GFP^low cells (HSPCs, white arrows) in the CHT region of cd41:EGFP transgenic WT and apoc2 mutants at 54 and 80 hpf (n = 10 in WT and n = 8 in apoc2 mutant groups at 54 hpf; n = 6 in each group at 80 hpf). e, f Representative images and numbers of GFP-positive cells in the thymus region at 54 hpf (n = 9 in WT and n = 8 in apoc2 mutant groups) and at 4 dpf (n = 8 in each group). Scale bars, 200 μm in a and b; 50 μm in c–f. Mean ± SEM; *P < 0.05 and ***P < 0.001 (Student’s t test)

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.