FIGURE

Fig. 9

ID

ZDB-FIG-180516-19

Publication

Ojeda Naharros et al., 2017 - Loss-of-function of the ciliopathy protein Cc2d2a disorganizes the vesicle fusion machinery at the periciliary membrane and indirectly affects Rab8-trafficking in zebrafish photoreceptors

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Fig. 9

SNAP25 localizes to wt periciliary membrane and mislocalizes to accumulated vesicles of cc2d2a-/- PRs (A-E) 5 dpf CLEM retinal sections stained for SNAP25 (green), acetylated tubulin (magenta) and DAPI (blue, nuclei). (C-E) are higher magnification images of the boxed areas in (A, B) and (C’-E’) are the corresponding SEM images. In wt PRs (A) SNAP25 is found at the inner segment apical membrane, along the calycal processes (empty arrowhead in A) as well as at the periciliary membrane (C-C’, arrow) around the base of the anti-acetylated tubulin-marked cilium (C-C’, arrowhead). In contrast, in cc2d2a-/- PRs (B), SNAP25 prominently mislocalizes in accumulated vesicles (D-D’, arrows) and dysmorphic OSs (E-E’, bracket). Arrowheads point to connecting cilia in (C-D’). Scale bars: 4 μm in A-B, 1 μm in C-E’. OS outer segment, m mitochondria, N nuclei, P pigment, v vesicles, wt wild-type.

Expression Data

Antibodies:	Ab1-actb Ab4-snap25
Fish:	WT cc2d2a^w38/w38
Anatomical Terms:	eye photoreceptor cell periciliary membrane compartment eye photoreceptor cell photoreceptor cell cilium eye photoreceptor cell photoreceptor inner segment eye photoreceptor cell photoreceptor inner segment membrane eye photoreceptor cell photoreceptor outer segment eye photoreceptor cell vesicle
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cc2d2a^w38/w38
Observed In:	eye photoreceptor cell photoreceptor outer segment eye photoreceptor cell vesicle photoreceptor cell apical region photoreceptor cell photoreceptor outer segment photoreceptor cell vesicle
Stage:	Day 5

Phenotype Detail

Acknowledgments

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