FIGURE

Fig. 5

ID

ZDB-FIG-180516-16

Publication

Ojeda Naharros et al., 2017 - Loss-of-function of the ciliopathy protein Cc2d2a disorganizes the vesicle fusion machinery at the periciliary membrane and indirectly affects Rab8-trafficking in zebrafish photoreceptors

Other Figures

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Fig. 5

Rab8a partially mislocalizes in cc2d2a-/- cones 5 dpf CLEM images of wild-type (wt) (A-A’) and cc2d2a-/- (B-C’) zebrafish expressing mCherry-tagged Rab8a in cones (tg(tacp:mCherry-rab8a), magenta). The mCherry signal is enhanced with anti-mCherry antibody. (A-A’) Note the localization of Rab8a (magenta) to vesicular structures (arrowheads) in wt. (B-B’) In cc2d2a-/- cones, Rab8a (magenta) localizes to accumulated vesicles (v, bracket) as well as to non-membrane-delimited cytoplasmic areas (arrows in C-C’). Scale bars are 3 μm in all panels. OS outer segment, m mitochondria, N nuclei, v vesicular structures, wt wild-type.

Expression Data

Gene:	mCherry
Fish:	mitfa^w2/w2; mpv17^a9/a9; zh403Tg cc2d2a^w38/w38; mitfa^w2/w2; mpv17^a9/a9; zh403Tg
Anatomical Terms:	retinal cone cell cytoplasm retinal cone cell vesicle
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cc2d2a^w38/w38; mitfa^w2/w2; mpv17^a9/a9; zh403Tg
Observed In:	photoreceptor cell apical region photoreceptor cell vesicle retinal cone cell cytoplasm
Stage:	Day 5

Phenotype Detail

Acknowledgments

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