ZFIN Image: Hui et al., 2017, Fig. 7

Image

Figure Caption

Fig. 7

Impaired Regeneration in Foxp3a-Deficient Zebrafish

(A) Foxp3a domain structure and predicted truncated product of the Foxp3a^vcc6 allele. Numbers indicate amino acid positions.

(B) Sections of Foxp3a-deficient (foxp3a^−/−) and WT (foxp3a^+/+) clutch-mate foxp3a:RFP fish. Asterisks indicate injury epicenter.

(C–E) Proliferation of newborn neurons (C), cardiomyocytes (D), and Müller glia (E). Insets show single-channel confocal slices (C and E) or epifluorescent images (D) of the demarcated regions. Arrows mark co-labeled cells. Dotted lines indicate the wound border.

(F–H) Quantification of (C), (D) and (E), respectively (mean ± SEM, n = 5–7).

(I–K) qRT-PCR analysis of regeneration factor genes (mean ± SEM, n = 6).

(L–N) Semi-qRT-PCR analysis of regeneration factor genes in purified foxp3a:RFP⁺ cells. +/+, foxp3a^+/+; −/−, foxp3a^−/−.

Confocal projections of z stacks are shown, except for the epifluorescent images in (D). ^∗p < 0.05, ^∗∗p < 0.01, Mann-Whitney U test. Scale bars, 50 μm. See also Figure S7.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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Reprinted from Developmental Cell, 43, Hui, S.P., Sheng, D.Z., Sugimoto, K., Gonzalez-Rajal, A., Nakagawa, S., Hesselson, D., Kikuchi, K., Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs, 659-672.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell