ZFIN ID: ZDB-FIG-180403-60
Norris et al., 2017 - Toddler signaling regulates mesodermal cell migration downstream of Nodal signaling. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage: 75%-epiboly

Fig. 7

Endodermal cell migration does not require Toddler signaling in cxcr4a mutants.

(A–B) Analysis of lateral mesodermal cell migration defects in cxcr4a mutant embryos by in situ hybridization for fn1a. Mesodermal cell migration is normal in stable cxcr4a single mutants. toddler;cxcr4a double mutants show mesodermal cell migration defects that resemble toddler single mutant siblings. (C) Representative images of embryos analyzed in D and E. In situ hybridization for sox17 at 75% epiboly; dorsal to the right. Cxcr4a morphants and cxcr4a mutants have excessive animal pole-directed migration of vegetal endoderm. Endoderm patterning in toddler;cxcr4a double mutants resembles wild-type embryos more than toddler single mutant siblings. (D) Measurement of frequency with which cells were found at a given location in an embryo of a certain genotype. A cell at the animal pole corresponds to 100% embryo height, while a cell at the vegetal pole corresponds to 0%. AP = Animal pole=100%; VP = vegetal pole=0%. (E) The number of lateral endodermal cells is unchanged between toddler single and toddler;cxcr4a double mutants. Each point represents a single embryo. Red bars are averages. (B and E) ns: p>0.46; *p<0.05; **p<0.0005; unpaired two-tailed t-test. N = number of independent experiments; n = number of embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fn1a WT standard conditions 75%-epiboly blastoderm ISH
apelaa141/a141 standard conditions 75%-epiboly blastoderm ISH
apelaa141/a141 ; cxcr4aa169/a169 standard conditions 75%-epiboly blastoderm ISH
apelaa142/a142 standard conditions 75%-epiboly blastoderm ISH
apelaa142/a142 ; cxcr4aa169/a169 standard conditions 75%-epiboly blastoderm ISH
cxcr4aa169/a169 standard conditions 75%-epiboly blastoderm ISH
sox17 WT standard conditions 75%-epiboly endoderm ISH
apelaa141/a141 standard conditions 75%-epiboly endoderm ISH
apelaa141/a141 ; cxcr4aa169/a169 standard conditions 75%-epiboly endoderm ISH
apelaa142/a142 standard conditions 75%-epiboly endoderm ISH
apelaa142/a142 ; cxcr4aa169/a169 standard conditions 75%-epiboly endoderm ISH
cxcr4aa169/a169 standard conditions 75%-epiboly blastoderm ISH
75%-epiboly endoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apelaa141/a141 standard conditions 75%-epiboly blastoderm fn1a expression spatial pattern, abnormal
75%-epiboly endoderm sox17 expression decreased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly mesodermal cell migration decreased process quality, abnormal
apelaa141/a141 ; cxcr4aa169/a169 standard conditions 75%-epiboly blastoderm fn1a expression spatial pattern, abnormal
75%-epiboly endoderm sox17 expression decreased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly endoderm cell migration involved in gastrulation process quality, abnormal
75%-epiboly mesodermal cell migration decreased process quality, abnormal
apelaa142/a142 standard conditions 75%-epiboly blastoderm fn1a expression spatial pattern, abnormal
75%-epiboly endoderm sox17 expression decreased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly mesodermal cell migration decreased process quality, abnormal
apelaa142/a142 ; cxcr4aa169/a169 standard conditions 75%-epiboly blastoderm fn1a expression spatial pattern, abnormal
75%-epiboly endoderm sox17 expression decreased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly endoderm cell migration involved in gastrulation process quality, abnormal
75%-epiboly mesodermal cell migration decreased process quality, abnormal
cxcr4aa169/a169 standard conditions 75%-epiboly endoderm sox17 expression decreased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly endoderm cell migration involved in gastrulation process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife