ZFIN ID: ZDB-FIG-180403-54
Norris et al., 2017 - Toddler signaling regulates mesodermal cell migration downstream of Nodal signaling. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2

Mesodermal cell migration and larval phenotypes are not rescued by increased numbers of endodermal cells.

(A) Schematic representation of experimental measurements shown in B. Densely packed, fn1a + lateral mesodermal cells are viewed by cross section. Location of the most animally migrated lateral cells of individual embryos are measured relative to AP-VP axis, then consolidated across embryos. AP = Animal pole; VP = vegetal pole. (B) Defects in animal-pole directed migration of mesodermal cells are still present in toddler;lefty2 double mutants. Each point represents a single embryo. Red bars are averages. p-Values for pairwise comparison with wild type unless indicated otherwise. *p=0.04, **p<0.001; ns: p=0.08; unpaired two-tailed t-test. (C–D) toddler;lefty2 double mutants resemble toddler single mutant siblings in respect to heart phenotypes at 2 days post fertilization (dpf). Hearts were classified as small if shortened by more than half of normal length and/or excessively narrow or thin. Hearts that were neither thin nor short but appeared to have looping defects or other patterning defects were classified as normal. Heart phenotypes were scored blind to genotype for toddler and toddler;lefty2 mutants. (C) Representative images of phenotypic classes after in situ hybridization for myl7. (D) Quantification of C. (E–F) toddler;lefty2 double mutants are more poorly patterned than toddler single mutant siblings in respect to tail phenotypes at 3 dpf. (E) Representative images of phenotypic classes. Phenotype classes II-V lack circulation. Arrow: accumulated blood. Arrowhead: defects in mesenchyme. * Duplicated tail tip. (F) Quantification of E. (B,D,F) N = number of independent experiments; n = number of embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 WT standard conditions Long-pec heart ISH
apelaa141/a141 standard conditions Long-pec heart ISH
apelaa142/a142 standard conditions Long-pec heart ISH
lft2a146/a146 ; apelaa141/a141 standard conditions Long-pec heart ISH
lft2a146/a146 ; apelaa142/a142 standard conditions Long-pec heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apelaa141/a141 standard conditions Long-pec heart decreased size, abnormal
Long-pec heart decreased width, abnormal
Protruding-mouth blood increased accumulation caudal fin, abnormal
Protruding-mouth caudal fin blood circulation decreased occurrence, abnormal
Protruding-mouth caudal fin mesenchyme morphology, abnormal
Protruding-mouth caudal fin morphology, abnormal
Protruding-mouth ventral fin fold mesenchyme morphology, abnormal
apelaa142/a142 standard conditions Long-pec heart decreased size, abnormal
Long-pec heart decreased width, abnormal
Protruding-mouth blood increased accumulation caudal fin, abnormal
Protruding-mouth caudal fin blood circulation decreased occurrence, abnormal
Protruding-mouth caudal fin mesenchyme morphology, abnormal
Protruding-mouth caudal fin morphology, abnormal
Protruding-mouth ventral fin fold mesenchyme morphology, abnormal
lft2a146/a146 ; apelaa141/a141 standard conditions Long-pec heart decreased size, abnormal
Long-pec heart decreased width, abnormal
Protruding-mouth blood increased accumulation caudal fin, abnormal
Protruding-mouth caudal fin blood circulation decreased occurrence, abnormal
Protruding-mouth caudal fin mesenchyme morphology, abnormal
Protruding-mouth caudal fin morphology, abnormal
Protruding-mouth ventral fin fold mesenchyme morphology, abnormal
lft2a146/a146 ; apelaa142/a142 standard conditions Long-pec heart decreased size, abnormal
Long-pec heart decreased width, abnormal
Protruding-mouth blood increased accumulation caudal fin, abnormal
Protruding-mouth caudal fin blood circulation decreased occurrence, abnormal
Protruding-mouth caudal fin mesenchyme morphology, abnormal
Protruding-mouth caudal fin morphology, abnormal
Protruding-mouth ventral fin fold mesenchyme morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife