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ZFIN ID: ZDB-FIG-180403-52
Norris et al., 2017 - Toddler signaling regulates mesodermal cell migration downstream of Nodal signaling. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage: 75%-epiboly

Fig. 1

Increased endodermal specification does not rescue endodermal cell migration in toddler;lefty2 double mutants.

(A) Representative images of embryos analyzed in B and D. In situ hybridization with a sox17 probe at 75% epiboly; dorsal to the right. Endoderm in toddler;lefty2 double mutants resembles toddler single mutants. (B) The number of lateral endodermal cells is increased in toddler;lefty2 double mutants compared to wild-type and toddler single mutant embryos. Each point represents a single embryo. Red bars are averages. p-Values for pairwise comparison with wild type unless otherwise noted. **p<0.001; unpaired two-tailed t-test. N = number of independent experiments; n = number of embryos. (C) Schematic representation of experimental measurements shown in B and D. The locations of lateral endodermal cells in individual embryos are measured relative to the AP-VP axis and then consolidated across embryos. (D) Measurement of frequency with which cells were found at a given location in an embryo of a certain genotype. A cell at the animal pole corresponds to 100% embryo height, while a cell at the vegetal pole corresponds to 0%. AP = Animal pole; VP = vegetal pole. The same embryos were measured in B and D.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sox17 WT standard conditions 75%-epiboly endoderm ISH
apelaa141/a141 standard conditions 75%-epiboly endoderm ISH
apelaa142/a142 standard conditions 75%-epiboly endoderm ISH
75%-epiboly endodermal cell ISH
lft2a146/a146 standard conditions 75%-epiboly endoderm ISH
lft2a146/a146 ; apelaa141/a141 standard conditions 75%-epiboly endoderm ISH
lft2a146/a146 ; apelaa142/a142 standard conditions 75%-epiboly endoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apelaa141/a141 standard conditions 75%-epiboly endoderm sox17 expression decreased amount, abnormal
75%-epiboly endoderm sox17 expression decreased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly endodermal cell decreased amount, abnormal
apelaa142/a142 standard conditions 75%-epiboly endoderm sox17 expression decreased amount, abnormal
75%-epiboly endoderm sox17 expression decreased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly endodermal cell decreased amount, abnormal
lft2a146/a146 standard conditions 75%-epiboly endoderm sox17 expression increased amount, abnormal
75%-epiboly endoderm sox17 expression increased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly endodermal cell increased amount, abnormal
lft2a146/a146 ; apelaa141/a141 standard conditions 75%-epiboly endoderm sox17 expression decreased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly endodermal cell increased amount, abnormal
lft2a146/a146 ; apelaa142/a142 standard conditions 75%-epiboly endoderm sox17 expression decreased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly endodermal cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife