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ZFIN ID: ZDB-FIG-180403-53
Norris et al., 2017 - Toddler signaling regulates mesodermal cell migration downstream of Nodal signaling. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: Shield to 75%-epiboly

Fig. 1-s1

Initial specification of endoderm is normal in toddler mutants.

(A–C) n = number of embryos (A) Number of lateral endodermal cells in wild-type and toddler mutant embryos during gastrulation. (B) Rate of endodermal cell division in wild-type and toddler mutant embryos. 40–60 cells were tracked per embryo and the percentage of those cells that divided is shown. Red bars are averages. (C) Percentage of embryos with a given number of TUNEL +cells on one side of the embryo. (D) Representative images of TUNEL staining at two time points in toddler mutants. (E) Live tracking of endoderm using sox17:GFP transgenic fish captures two endodermal cells undergoing cell death during gastrulation in toddler mutants (arrows). A cell that is about to divide is also present (asterisk).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ha01Tg ; apelaa141/a141 standard conditions 75%-epiboly endodermal cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apelaa141/a141 standard conditions 50%-epiboly endoderm apoptotic process increased occurrence, abnormal
Shield endoderm apoptotic process increased occurrence, abnormal
75%-epiboly endoderm apoptotic process increased occurrence, abnormal
apelaa142/a142 standard conditions Shield endoderm apoptotic process increased occurrence, abnormal
50%-epiboly endoderm apoptotic process increased occurrence, abnormal
75%-epiboly endoderm apoptotic process increased occurrence, abnormal
ha01Tg ; apelaa141/a141 standard conditions 75%-epiboly endodermal cell apoptotic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife