ZFIN ID: ZDB-FIG-180403-57
Norris et al., 2017 - Toddler signaling regulates mesodermal cell migration downstream of Nodal signaling. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: 50%-epiboly to 75%-epiboly

Fig. 5

toddler mutant mesodermal cells have diminished animal pole-directed migration during gastrulation.

(A–B) Cas9-mediated mutagenesis was used to generate sox32 mutants. Embryos were injected at the one-cell stage either with sox32 mRNA or with Cas9 + sox32 gRNAs. in situ hybridization for sox17 and aplnrB at shield stage; dorsal to the right. (A) Expression of aplnrB decreases in the presence of excess endoderm and increases in the absence of endoderm. (B) Cross-section of shield stage embryo. The majority of cells expressing aplnrB in embryos lacking endoderm are internalized and unlikely to be ectodermal. Arrow: internalized cells. Arrowhead: ectoderm. (C–E) Mesodermal cell migration was tracked during gastrulation using a drl:eGFP transgene and lightsheet microscopy. drl:eGFP labels ventrolateral mesoderm during gastrulation. toddler mutant measurements represent three embryos. Wild-type measurements represent two wild-type and one heterozygous embryos. (C) Representative still frames of maximum intensity projections from a wild-type and toddler mutant drl:eGFP transgenic embryo. See also Video 1. (D–E) Movies spanning 50% to 85% epiboly were aligned at the onset of internalization and binned into 45 min windows. (D) Measurement of straightness and velocity of mesodermal cells in wild-type and toddler mutants. Straightness is defined as the difference between the total movement of a cell in all directions divided by the net displacement (the actual distance between the cell’s beginning and ending location). (E) toddler mutant cells have diminished migration animally during gastrulation. Each bin represents the proportion of cells moving in a given direction weighted by the total distance traveled in that direction. n = number of cells. A = animal, D = dorsal, Vg = vegetal, V = ventral.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aplnrb WT standard conditions Shield blastoderm ISH
Shield margin ISH
EGFP apelaa141/a141 ; cz3331Tg standard conditions 50%-epiboly to 75%-epiboly mesodermal cell IFL
apelaa142/a142 ; cz3331Tg standard conditions 50%-epiboly to 75%-epiboly mesodermal cell IFL
cz3331Tg standard conditions 50%-epiboly to 75%-epiboly mesodermal cell IFL
sox17 WT standard conditions Shield blastoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apelaa141/a141 ; cz3331Tg standard conditions 50%-epiboly - 75%-epiboly mesodermal cell migration decreased process quality, abnormal
apelaa142/a142 ; cz3331Tg standard conditions 50%-epiboly - 75%-epiboly mesodermal cell migration decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife