ZFIN ID: ZDB-FIG-180403-55
Norris et al., 2017 - Toddler signaling regulates mesodermal cell migration downstream of Nodal signaling. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: 50%-epiboly

Fig. 3

Wild-type and toddler mutant embryos have similar levels of Nodal signaling.

(A) Representative images of Nodal target gene expression in wild-type and toddler mutant embryos based on in situ hybridization at 50% epiboly. Animal pole facing up. Three biological replicates. (B–C) aplnrA;aplnrB double heterozygote parents were incrossed and their progeny examined by in situ hybridization at 50% epiboly. (B) Representative images of Nodal target gene expression in offspring from aplnrA;aplnrB double heterozygote parents. Animal pole facing up. (C) All embryos from B were genotyped. Results for relevant genotypes are shown. Expression of lefty1 and lefty2 in aplnrA;aplnrB double mutant embryos was indistinguishable from wild type. (D) RNA-sequencing before and during early gastrulation reveals no differential transcription of Nodal targets (black dots) between wild-type and toddler mutant embryos. All other genes are shaded in light gray. See also Figure 3—Source data 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lft1 WT standard conditions 50%-epiboly blastoderm ISH
apelaa141/a141 standard conditions 50%-epiboly blastoderm ISH
apelaa142/a142 standard conditions 50%-epiboly blastoderm ISH
aplnramu296/mu296 ; aplnrba170/a170 standard conditions 50%-epiboly blastoderm ISH
lft2 WT standard conditions 50%-epiboly blastoderm ISH
apelaa141/a141 standard conditions 50%-epiboly blastoderm ISH
apelaa142/a142 standard conditions 50%-epiboly blastoderm ISH
aplnramu296/mu296 ; aplnrba170/a170 standard conditions 50%-epiboly blastoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife