FIGURE

Fig. 3

ID

ZDB-FIG-180312-4

Publication

Li et al., 2017 - Rapid functional analysis of computationally complex rare human IRF6 gene variants using a novel zebrafish model

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Maternal-null irf6^-/- embryo gene expression is rescued by injection of either zebrafish or human wild type IRF6 mRNA.

(A-H) Whole-mount in situ hybridization analysis of wild type embryos (A-D) compared to maternal/zygotic-null irf6 ^-/- embryos (E-H) at the sphere stage revealed strong down-regulation of critical irf6 downstream genes such as krt4 and grhl3. Scale bar = 150 μm. (I) Relative gene expression of wild type embryos, maternal/zygotic-null irf6 ^-/- embryos, and maternal/zygotic-null irf6 ^-/- embryos rescued with either wild type zebrafish or human IRF6 mRNA (100 pg) microinjections, for a panel of genes with crucial roles in the irf6 gene regulatory network. Error bar = 2xSEM, n = 3.

Expression Data

Genes:	cebpb cldne gata3 grhl1 grhl3 hey1 klf2a krt4 krt18a.1 tfap2a
Fish:	TU irf6^zf2064 (TU)
Anatomical Term:	whole organism
Stage:	Sphere

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	irf6^zf2064 (TU)
Observed In:	whole organism
Stage:	Sphere

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.