FIGURE

Fig. 3

ID
ZDB-FIG-180312-4
Publication
Li et al., 2017 - Rapid functional analysis of computationally complex rare human IRF6 gene variants using a novel zebrafish model
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Fig. 3

Maternal-null irf6-/- embryo gene expression is rescued by injection of either zebrafish or human wild type IRF6 mRNA.

(A-H) Whole-mount in situ hybridization analysis of wild type embryos (A-D) compared to maternal/zygotic-null irf6 -/- embryos (E-H) at the sphere stage revealed strong down-regulation of critical irf6 downstream genes such as krt4 and grhl3. Scale bar = 150 μm. (I) Relative gene expression of wild type embryos, maternal/zygotic-null irf6 -/- embryos, and maternal/zygotic-null irf6 -/- embryos rescued with either wild type zebrafish or human IRF6 mRNA (100 pg) microinjections, for a panel of genes with crucial roles in the irf6 gene regulatory network. Error bar = 2xSEM, n = 3.

Expression Data
Genes:
Fish:
Anatomical Term:
Stage: Sphere

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Sphere

Phenotype Detail
Acknowledgments
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