Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-180126-9
Facchinello et al., 2017 - Tcf7l2 plays pleiotropic roles in the control of glucose homeostasis, pancreas morphology, vascularization and regeneration. Scientific Reports   7:9605 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Days 7-13 to Adult

Fig. 2

Canonical Wnt signalling and tcf7l2 expression in the pancreas. (A,B) Single and double fluorescent WISH comparing the expression of tcf7l2 and insulin at 7 dpf. Images show confocal single planes at 7 dpf at different magnification of (A) (20x) and (B,B’,B”) (60x). Merging the green and red channels identifies regions with distinct gene expression of the two genes (B’,B”). in: intestine, e: exocrine tissues, i: principal islet. Scale bar = 100 μm. (C) Expression level (count normalized by library size) obtained by RNAseq of different Tcf/Lef genes in α, β, δ and acinar cells from adult zebrafish. (D) tcf7l2 and egfp expression values from 10 different tissues derived from transgenic Tg(fli1a:EGFP)y1 adult fish. Brain (Br), Eye (Ey), Gills (Gi), Gut (Gu), Heart (He), Kidney (Ki), Muscle (Mu), Pancreas (Pa), Vessels (Ve). (E) Enrichment of GFP+ cells from Tg(fli1a:EGFP)y1 zebrafish embryos. Relative expression of indicated genes, in GFP+ (green bars) and GFP (grey bars) cells, determined by quantitative RT-PCR. Relative expression levels were determined by normalization to arp. Values represent the mean ± SEM. Asterisk above column indicate statistical differences among groups.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Adult blood vessel RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult gill RTPCR
Adult gut RTPCR
Adult heart RTPCR
Adult integument RTPCR
Adult kidney RTPCR
Adult muscle RTPCR
ins WT standard conditions Days 7-13 exocrine pancreas ISH
tcf7l1a gz18Tg standard conditions Adult pancreatic D cell RTPCR
ia1Tg standard conditions Adult pancreatic A cell RTPCR
jh1Tg standard conditions Adult pancreatic acinar cell RTPCR
m1015Tg standard conditions Adult pancreatic B cell RTPCR
tcf7l1b gz18Tg standard conditions Adult pancreatic D cell RTPCR
ia1Tg standard conditions Adult pancreatic A cell RTPCR
jh1Tg standard conditions Adult pancreatic acinar cell RTPCR
tcf7l2 WT standard conditions Days 7-13 exocrine pancreas ISH
Days 7-13 intestine ISH
Days 7-13 primary islet ISH
gz18Tg standard conditions Adult pancreatic D cell RTPCR
jh1Tg standard conditions Adult pancreatic acinar cell RTPCR
y1Tg standard conditions Adult blood vessel RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult gill RTPCR
Adult gut RTPCR
Adult heart RTPCR
Adult integument RTPCR
Adult kidney RTPCR
Adult muscle RTPCR
Adult pancreas RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.