ZFIN ID: ZDB-FIG-180126-10
Facchinello et al., 2017 - Tcf7l2 plays pleiotropic roles in the control of glucose homeostasis, pancreas morphology, vascularization and regeneration. Scientific Reports   7:9605 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Days 7-13 to Days 14-20
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 7-13 to Days 14-20

Fig. 3

tcf7l2 is required for regulation of insulin expression and exocrine pancreas development. Analysis of insulin in control embryos (A) and in tcf7l2 exI/exI mutants (A’) by in situ hybridization. Lateral views of the pancreatic area are shown with the anterior side to the right. The expression of insulin is significantly reduced in the mutants. (B–B”) Analysis of pancreatic islet in tcf7l2 exI/exI mutant in Tg(ins:dsRed) background. 2D projections of confocal Z-series images of DsRed expression in living Tg(ins:DsRed) embryos at 16 dpf. (B) wt; (B’) homozygous mutant. (C) Graphic presentation of the integrated density of fluorescence in the red channel in tcf7l2 exI/exI mutant and wild-type sib controls in Tg(ins:dsRed) at 16 dpf. (D) Quantification of the number of β cells during juvenile growth of tcf7l2 exI/exI and control siblings. (E–E’) Analysis of exocrine pancreas in tcf7l2 exI/exI mutant in Tg(ptf1a:dsRed), (E) wt and (E’) mutant at 16 dpf. (F) Graphic presentation of the integrated density of fluorescence in the red channel in tcf7l2 exI/exI mutant and wild-type sib controls in Tg(ela3l:Crimson) at 7 and 14 dpf. Data were obtained from 6 individuals per genotype, repeated in 2 different experiments. All reference to phenotypes was confirmed by genotyping. The integrated density was obtained using the Fiji software. Values represent the mean ± SEM. Asterisk above column indicate statistical differences among groups *p < 0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Crimson ml19Tg control Days 7-13 exocrine pancreas IFL
Days 14-20 exocrine pancreas IFL
ml19Tg ; tcf7l2zf55/zf55 standard conditions Days 7-13 exocrine pancreas IFL
Days 14-20 exocrine pancreas IFL
DsRed ia6Tg control Days 14-20 exocrine pancreas IFL
ia6Tg ; tcf7l2zf55/zf55 standard conditions Days 14-20 exocrine pancreas IFL
m1018Tg control Days 14-20 pancreas IFL
tcf7l2zf55/zf55 ; m1018Tg standard conditions Days 14-20 pancreas IFL
tcf7l2 WT control Days 7-13 pancreas ISH
tcf7l2zf55/zf55 standard conditions Days 7-13 pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ia6Tg ; tcf7l2zf55/zf55 control Days 14-20 exocrine pancreas morphology, abnormal
ia6Tg ; tcf7l2zf55/zf55 standard conditions Days 14-20 exocrine pancreas DsRed expression decreased amount, abnormal
ml19Tg ; tcf7l2zf55/zf55 standard conditions Days 14-20 exocrine pancreas Crimson expression decreased amount, abnormal
tcf7l2zf55/zf55 standard conditions Days 7-13 pancreas tcf7l2 expression decreased amount, abnormal
tcf7l2zf55/zf55 ; m1018Tg standard conditions Days 14-20 blood glucose increased amount, abnormal
Days 14-20 pancreas DsRed expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.