ZFIN ID: ZDB-FIG-180123-27
Yan et al., 2017 - CD146 is required for VEGF-C-induced lymphatic sprouting during lymphangiogenesis. Scientific Reports   7:7442 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-25 to Protruding-mouth

Fig. 5

CD146 modulates VEGF-C induced lymphatic development in zebrafish. (a) CD146 expression in the zebrafish embryos at 5 dpf was detected by whole-mount in situ hybridization (WISH). lyve1b was used as positive control. Black arrows indicate the position of TD. DA, dorsal aorta; PCV, posterior cardinal vein; and TD, thoracic duct. Scale bars: 30 μm. (b) The effect of knockdown cd146 or vegfc on blood vessels. Bright-field lateral views of the trunk of embryos injected with control, cd146 or vegfc specific MOs. Embryos were stained by WISH for a panel of arterial markers of dll4, tbx20 and ephrinB2 and venous markers of msr and flt4. Black arrows, blood vessels; DA, dorsal aorta; and PCV, posterior cardinal vein. Scale bars: 125 μm. (c) The effect of knockdown cd146 or vegfc on vasculature of blood and lymph. The control, cd146 or vegfc specific MO were injected into the transgenic zebrafish Tg (fli1a: EGFP) embryos, respectively. The embryos harvested at 36 hpf, 48 hpf and 72 hpf were analyzed. The confocal images were flanked by redrawing of the vessel contours. Transient lymphangiogenic structures (lymphangiogenic sprouts; PL cells) were labeled with light green. The other vessels (PCV, DA and ISV) were labeled with dark grey. Scale bars: 20 μm. (d) The effects of cd146 knockdown on development of lymphatic capillary. The control or cd146 specific MO was co-injected with control or human vegfc mRNA into the transgenic zebrafish Tg (fli1a: EGFP) embryos. Late-phase microangiography was used to visualize lymphatic capillaries in embryos at 3 dpf. Images showed the representative phenotypes of each group and the ratio indicated the embryos with such representative phenotype over the tested embryos. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aplnra TU standard conditions Prim-5 trunk vein ISH
TU + MO1-vegfc standard conditions Prim-5 trunk vein ISH
TU + MO3-mcamb standard conditions Prim-5 trunk vein ISH
dll4 TU standard conditions Prim-5 trunk artery ISH
TU + MO1-vegfc standard conditions Prim-5 trunk artery ISH
TU + MO3-mcamb standard conditions Prim-5 trunk artery ISH
efnb2a TU standard conditions Prim-25 trunk artery ISH
TU + MO1-vegfc standard conditions Prim-25 trunk artery ISH
TU + MO3-mcamb standard conditions Prim-25 trunk artery ISH
EGFP y1Tg standard conditions Prim-25 dorsal aorta IFL
Prim-25 intersegmental vessel IFL
Prim-25 lymphangiogenic sprout IFL
Prim-25 posterior cardinal vein IFL
Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec lymphangiogenic sprout IFL
Long-pec posterior cardinal vein IFL
Protruding-mouth dorsal aorta IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth lymphangiogenic sprout IFL
Protruding-mouth posterior cardinal vein IFL
y1Tg + MO1-vegfc standard conditions Prim-25 dorsal aorta IFL
Prim-25 intersegmental vessel IFL
Prim-25 lymphangiogenic sprout IFL
Prim-25 posterior cardinal vein IFL
Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec lymphangiogenic sprout IFL
Long-pec posterior cardinal vein IFL
Protruding-mouth dorsal aorta IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth lymphangiogenic sprout IFL
Protruding-mouth posterior cardinal vein IFL
y1Tg + MO3-mcamb standard conditions Prim-25 dorsal aorta IFL
Prim-25 intersegmental vessel IFL
Prim-25 lymphangiogenic sprout IFL
Prim-25 posterior cardinal vein IFL
Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec lymphangiogenic sprout IFL
Long-pec posterior cardinal vein IFL
Protruding-mouth dorsal aorta IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth lymphangiogenic sprout IFL
Protruding-mouth posterior cardinal vein IFL
flt4 TU standard conditions Prim-5 trunk vein ISH
TU + MO1-vegfc standard conditions Prim-5 trunk vein ISH
TU + MO3-mcamb standard conditions Prim-5 trunk vein ISH
lyve1b TU standard conditions Day 5 thoracic duct ISH
mcamb TU standard conditions Day 5 thoracic duct ISH
tbx20 TU standard conditions Prim-5 trunk artery ISH
TU + MO1-vegfc standard conditions Prim-5 trunk artery ISH
TU + MO3-mcamb standard conditions Prim-5 trunk artery ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-vegfc standard conditions Prim-25 lymphangiogenesis decreased occurrence, abnormal
Prim-25 lymphangiogenic sprout decreased amount, abnormal
Long-pec lymphangiogenesis decreased occurrence, abnormal
Long-pec lymphangiogenic sprout decreased amount, abnormal
Protruding-mouth lymphangiogenesis decreased occurrence, abnormal
Protruding-mouth lymphangiogenic sprout decreased amount, abnormal
y1Tg + MO3-mcamb standard conditions Prim-25 lymphangiogenesis decreased occurrence, abnormal
Prim-25 lymphangiogenic sprout decreased amount, abnormal
Long-pec lymphangiogenesis decreased occurrence, abnormal
Long-pec lymphangiogenic sprout decreased amount, abnormal
Protruding-mouth lymphangiogenesis decreased occurrence, abnormal
Protruding-mouth lymphangiogenic sprout decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.