ZFIN ID: ZDB-FIG-180123-28
Yan et al., 2017 - CD146 is required for VEGF-C-induced lymphatic sprouting during lymphangiogenesis. Scientific Reports   7:7442 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Pec-fin to Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Pec-fin to Day 5

Fig. 6

Knockdown of CD146 disrupts lymphangiogenesis in zebrafish. (a,b) The effects of cd146 or vegfc knockdown on PL formation. Tg (fli1a: EGFP) embryos, injected with control, cd146 or vegfc specific MO, were harvested at 60 hpf. Confocal images showed normal PL (red arrows) and impaired PL (red stars) in (a). Scale bars: 50 μm. The percentages of embryos with normal PL cells (normal) and impaired PL string formation (impaired PLs) were shown in (b). (c,d) The effects of cd146 or vegfc knockdown on TD formation. Tg (fli1a: EGFP) embryos, injected with control, cd146 or vegfc specific MO, were harvested at 5 dpf. Confocal images showed normal TD (white arrows) and abnormal TD (white stars) in (c). Scale bars: 30 μm. The percentages of embryos with different phenotypes of TD formation were shown in (d). Relative to the normal embryos, the TD length decreased to 30–90% were classified as weak, and those decreased to 10–30% were classified as severe. (e,f) The effects of cd146 knockdown on TD function. Tg (flk1: mcherry) zebrafish embryos injected with control or cd146 specific MO and FITC-dextran was injected subcutaneously in the tail of those fishes. The embryos were harvested at 5 dpf. Confocal images showed functional TD (yellow arrows) and dysfunctional TD (yellow stars) in (e). Scale bars: 50 μm. The percentages of embryos with different phenotypes of TD formation were shown in (f). The criterion used to classify the TD formation was the same as that used in (d). In all panels, the head of the embryo faces left and dorsal is up.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Pec-fin dorsal aorta IFL
Pec-fin intersegmental vessel IFL
Pec-fin lymphangiogenic sprout IFL
Pec-fin posterior cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 intersegmental vessel IFL
Day 5 lymphangiogenic sprout IFL
Day 5 posterior cardinal vein IFL
y1Tg + MO1-vegfc standard conditions Pec-fin dorsal aorta IFL
Pec-fin intersegmental vessel IFL
Pec-fin posterior cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 intersegmental vessel IFL
Day 5 posterior cardinal vein IFL
y1Tg + MO3-mcamb standard conditions Pec-fin dorsal aorta IFL
Pec-fin intersegmental vessel IFL
Pec-fin posterior cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 intersegmental vessel IFL
Day 5 posterior cardinal vein IFL
mCherry ci5Tg standard conditions Day 5 dorsal aorta IFL
Day 5 posterior cardinal vein IFL
ci5Tg + MO3-mcamb standard conditions Day 5 dorsal aorta IFL
Day 5 posterior cardinal vein IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ci5Tg + MO3-mcamb standard conditions Day 5 thoracic duct decreased functionality, abnormal
Day 5 thoracic duct malformed, abnormal
y1Tg + MO1-vegfc standard conditions Pec-fin vascular lymphangioblast absent, abnormal
Pec-fin vascular lymphangioblast lymphangiogenesis decreased occurrence, abnormal
Day 5 thoracic duct absent, abnormal
Day 5 thoracic duct lymphangiogenesis decreased occurrence, abnormal
y1Tg + MO3-mcamb standard conditions Pec-fin vascular lymphangioblast absent, abnormal
Pec-fin vascular lymphangioblast lymphangiogenesis decreased occurrence, abnormal
Day 5 thoracic duct absent, abnormal
Day 5 thoracic duct decreased amount, abnormal
Day 5 thoracic duct decreased length, abnormal
Day 5 thoracic duct lymphangiogenesis decreased occurrence, abnormal
Day 5 thoracic duct malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.