ZFIN ID: ZDB-FIG-171205-26
Poureetezadi et al., 2016 - Prostaglandin signaling regulates nephron segment patterning of renal progenitors during zebrafish kidney development. eLIFE   5 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4

Prostaglandin signal inhibition results in an expansion of the distal early domain, which is rescued by the addition of exogenous dmPGE2.

Embryos were treated with a (A) 1% DMSO control, the (B) Ptgs1 selective inhibitor SC-560 at 50 μM from 4 hpf to 24 hpf, microinjected with the ptgs1 MO alone, or microinjected with the ptgs1 MO and treated with dmPGE2 from 4 hpf to 24 hpf. Embryos were treated with the (C) Ptgs2a selective inhibitor NS-398 at 50 μM from 4 hpf to 24 hpf, microinjected with the ptgs2a MO or microinjected with the ptgs2a MO and then treated with dmPGE2 at 50 μM from 4 hpf to 24 hpf. (D) Embryos were microinjected with a combination of the ptgs1 MO and ptgs2a MO. Embryos were microinjected with (E) ptger2a MO1, ptger2a MO2, (F) ptger4a MO1 and ptger4a MO2. (AE) WISH was used to stain for the DE (slc12a1), DL (slc12a3) (purple), and the somites (smyhc1) (red) at the 24 hpf stage. Black bars indicate segment gene expression domain. Red scale bar, 70 µm. (G) Summary depicting the nephron segments after inducing deficiency of prostaglandin synthesis or receptor activity.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
slc12a1 TU control Prim-5 pronephric distal early tubule ISH
TU chemical treatment by environment: NS-398 Prim-5 pronephric distal early tubule ISH
TU chemical treatment by environment: SC560 Prim-5 pronephric distal early tubule ISH
TU + MO1-ptger2a standard conditions Prim-5 pronephric distal early tubule ISH
TU + MO1-ptger2a chemical treatment by environment: 16,16-dimethylprostaglandin E2 Prim-5 pronephric distal early tubule ISH
TU + MO1-ptger2a + MO1-ptgs1 standard conditions Prim-5 pronephric distal early tubule ISH
TU + MO1-ptger4a standard conditions Prim-5 pronephric distal early tubule ISH
TU + MO1-ptgs1 standard conditions Prim-5 pronephric distal early tubule ISH
TU + MO1-ptgs1 chemical treatment by environment: 16,16-dimethylprostaglandin E2 Prim-5 pronephric distal early tubule ISH
TU + MO2-ptger2a standard conditions Prim-5 pronephric distal early tubule ISH
TU + MO2-ptger4a standard conditions Prim-5 pronephric distal early tubule ISH
slc12a3 TU control Prim-5 pronephric distal late tubule ISH
TU chemical treatment by environment: NS-398 Prim-5 pronephric distal late tubule ISH
TU chemical treatment by environment: SC560 Prim-5 pronephric distal late tubule ISH
TU + MO1-ptger2a standard conditions Prim-5 pronephric distal late tubule ISH
TU + MO1-ptger2a chemical treatment by environment: 16,16-dimethylprostaglandin E2 Prim-5 pronephric distal late tubule ISH
TU + MO1-ptger2a + MO1-ptgs1 standard conditions Prim-5 pronephric distal late tubule ISH
TU + MO1-ptger4a standard conditions Prim-5 intermediate mesoderm ISH
Prim-5 pronephric distal late tubule ISH
TU + MO1-ptgs1 standard conditions Prim-5 pronephric distal late tubule ISH
TU + MO1-ptgs1 chemical treatment by environment: 16,16-dimethylprostaglandin E2 Prim-5 pronephric distal late tubule ISH
TU + MO2-ptger2a standard conditions Prim-5 pronephric distal late tubule ISH
TU + MO2-ptger4a standard conditions Prim-5 pronephric distal late tubule ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment by environment: NS-398 Prim-5 pronephric distal early tubule slc12a1 expression increased distribution, abnormal
Prim-5 pronephric distal early tubule increased size, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric distal late tubule decreased size, abnormal
TU chemical treatment by environment: SC560 Prim-5 pronephric distal early tubule slc12a1 expression increased distribution, abnormal
Prim-5 pronephric distal early tubule increased size, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric distal late tubule decreased size, abnormal
TU + MO1-ptger2a standard conditions Prim-5 pronephric distal early tubule slc12a1 expression increased distribution, abnormal
Prim-5 pronephric distal early tubule increased size, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric distal late tubule decreased size, abnormal
TU + MO1-ptger2a chemical treatment by environment: 16,16-dimethylprostaglandin E2 Prim-5 pronephric distal early tubule slc12a1 expression spatial pattern, ameliorated
Prim-5 pronephric distal early tubule size, ameliorated
Prim-5 pronephric distal late tubule slc12a3 expression spatial pattern, ameliorated
Prim-5 pronephric distal late tubule size, ameliorated
TU + MO1-ptger2a + MO1-ptgs1 standard conditions Prim-5 pronephric distal early tubule slc12a1 expression increased distribution, abnormal
Prim-5 pronephric distal early tubule increased size, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric distal late tubule decreased size, abnormal
TU + MO1-ptger4a standard conditions Prim-5 pronephric distal early tubule slc12a1 expression increased distribution, abnormal
Prim-5 pronephric distal early tubule increased size, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric distal late tubule decreased size, abnormal
TU + MO1-ptgs1 standard conditions Prim-5 pronephric distal early tubule slc12a1 expression increased distribution, abnormal
Prim-5 pronephric distal early tubule increased size, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric distal late tubule decreased size, abnormal
TU + MO1-ptgs1 chemical treatment by environment: 16,16-dimethylprostaglandin E2 Prim-5 pronephric distal early tubule slc12a1 expression spatial pattern, ameliorated
Prim-5 pronephric distal early tubule size, ameliorated
Prim-5 pronephric distal late tubule slc12a3 expression spatial pattern, ameliorated
Prim-5 pronephric distal late tubule size, ameliorated
TU + MO2-ptger2a standard conditions Prim-5 pronephric distal early tubule slc12a1 expression increased distribution, abnormal
Prim-5 pronephric distal early tubule increased size, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric distal late tubule decreased size, abnormal
TU + MO2-ptger4a standard conditions Prim-5 pronephric distal early tubule slc12a1 expression increased distribution, abnormal
Prim-5 pronephric distal early tubule increased size, abnormal
Prim-5 pronephric distal late tubule slc12a3 expression decreased distribution, abnormal
Prim-5 pronephric distal late tubule decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife