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ZFIN ID: ZDB-FIG-170912-21
Lamont et al., 2016 - The LIM-homeodomain transcription factor Islet2a promotes angioblast migration. Developmental Biology   414:181-92 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2

Isl2 modulates vein cell marker expression. Compared to wild type controls (A,C,E,G), expression of the venous markers flt4 (B) and mrc1 (D) is reduced in the trunk of isl2a morphants at 24 hpf, while there is no obvious change in the expression of arterial markers notch3 (F) and ephrinb2 (H). (A′-H′) are cross sections of embryos in A-H. (I) There is a 41% reduction in flt4 expression by qPCR (p<0.0001 by student's t-test) in isl2a morphants. Scale bars represent 200 µm in A-H and 30 µm in cross-sections A′-H′. Abbreviations: posterior cardinal vein (pcv), dorsal aorta (da), neural tube (nt) and pronephric ducts (pd).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
efnb2a TL control Prim-5 dorsal aorta ISH
TL + MO4-isl2a + MO5-isl2a control Prim-5 dorsal aorta ISH
flt4 TL control Prim-5 posterior cardinal vein ISH
Prim-5 whole organism RTPCR
TL + MO4-isl2a + MO5-isl2a control Prim-5 posterior cardinal vein ISH
Prim-5 whole organism RTPCR
mrc1a TL control Prim-5 posterior cardinal vein ISH
TL + MO4-isl2a + MO5-isl2a control Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
notch3 TL control Prim-5 dorsal aorta ISH
Prim-5 neural tube ISH
Prim-5 pronephric duct ISH
TL + MO4-isl2a + MO5-isl2a control Prim-5 dorsal aorta ISH
Prim-5 neural tube ISH
Prim-5 pronephric duct ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO4-isl2a + MO5-isl2a control Prim-5 dorsal aorta mrc1a expression mislocalised, abnormal
Prim-5 posterior cardinal vein mrc1a expression decreased distribution, abnormal
Prim-5 posterior cardinal vein flt4 expression decreased distribution, abnormal
Prim-5 whole organism flt4 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 414, Lamont, R.E., Wu, C.Y., Ryu, J.R., Vu, W., Davari, P., Sobering, R.E., Kennedy, R.M., Munsie, N.M., Childs, S.J., The LIM-homeodomain transcription factor Islet2a promotes angioblast migration, 181-92, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.