ZFIN ID: ZDB-FIG-170206-18
Lu et al., 2017 - Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish. Developmental Biology   421(2):139-148 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 10-13 somites

Fig. 3

Quantification of cardiac progenitor proliferation and apoptosis in LA1110 mutants and wild type siblings. (A) Dorsal view of pHH3 immunostaining (red; nuclei) on a 10-somite stage wild type embryo. The cardiac progenitor cells are visualized by nkx2.5 fluorescent in situ hybridization (green; cytoplasmic). Orthogonal reconstructions are shown on the bottom and the right side of the image. (B) Graph of the average number of pHH3-positive cardiac progenitor cells in wild type (n=9) and LA1110 mutant embryos (n=7). (C) Dorsal view of apoptotic bodies (red) detected by TUNEL assay in a 10-somite stage wild type embryo. Cardiac progenitor cells are marked by nkx2.5 fluorescent in situ hybridization (green; cytoplasmic). Orthogonal reconstructions are shown on the bottom and the right side of the image. (D) Graph of the average number of apoptotic bodies in the cardiac progenitor region in wild type (n=5) and LA1110 mutant embryos (n=5). Error bars indicate standard deviations. ns, not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nkx2.5 WT control 10-13 somites anterior lateral plate mesoderm heart primordium ISH
tbx20la1110/la1110 control 10-13 somites anterior lateral plate mesoderm heart primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx20la1110/la1110 control 10-13 somites anterior lateral plate mesoderm heart primordium nkx2.5 expression decreased amount, abnormal
10-13 somites heart primordium apoptotic process occurrence, normal
10-13 somites heart primordium cell population proliferation occurrence, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 421(2), Lu, F., Langenbacher, A., Chen, J.N., Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish, 139-148, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.