FIGURE

Fig. 6

ID

ZDB-FIG-170206-21

Publication

Lu et al., 2017 - Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish

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Fig. 6

Tbx20 overexpression significantly increases cardiomyocyte number. (A-H) Dorsal views of nkx2.5, mef2ca, scl and myl7 expression in non-transgenic (A, C, E and G) and Tg(hsp70l:Tbx20) embryos (B, D, F and H). The expression of nkx2.5 and mef2ca is expanded in Tbx20-overexpressing embryos at the 10-somite stage (A-D), whereas the expression of scl is similar to that of control embryos (E and F). Similarly, myl7 expression is enhanced in Tg(hsp70l:Tbx20) embryos at the 16-somite stage (H). (I and J) Representative confocal projections of myl7:nucGFP hearts from heat-treated non-transgenic (I) and Tg(hsp70l:Tbx20) embryos (J) at 40hpf. (K) Graph of the average number of nucGFP-positive cardiomyocytes in heat-treated non-transgenic (n=8) and Tg(hsp70l:Tbx20) embryos (n=10). Tbx20 overexpression enhanced cardiomyocyte production. (L) Graph of the ratio of BrdU-positive cardiomyocytes to the total number of cardiomyocytes in heat-treated non-transgenic and Tg(hsp70l:Tbx20) embryos (n=7). Overexpression of Tbx20 in early embryos promoted cardiomyocyte proliferation. Error bars indicate standard deviations. Asterisks indicate a significant difference (**p<0.01, ***p<0.001).

Expression Data

Genes:	EGFP mef2ca myl7 nkx2.5 tal1
Fish:	WT chb2Tg la2368Tg chb2Tg; la2368Tg
Condition:	heat shock
Anatomical Terms:	anterior lateral plate mesoderm heart primordium anterior lateral plate mesoderm hematopoietic cell cardiac muscle cell cardiac muscle cell nucleus
Stage Range:	10-13 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la2368Tg chb2Tg; la2368Tg
Condition:	heat shock
Observed In:	anterior lateral plate mesoderm heart primordium cardiac muscle cell cardiac muscle cell cell population proliferation
Stage Range:	10-13 somites to Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 421(2), Lu, F., Langenbacher, A., Chen, J.N., Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish, 139-148, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.