ZFIN ID: ZDB-FIG-170206-21
Lu et al., 2017 - Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish. Developmental Biology   421(2):139-148 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 10-13 somites to Prim-25

Fig. 6

Tbx20 overexpression significantly increases cardiomyocyte number. (A-H) Dorsal views of nkx2.5, mef2ca, scl and myl7 expression in non-transgenic (A, C, E and G) and Tg(hsp70l:Tbx20) embryos (B, D, F and H). The expression of nkx2.5 and mef2ca is expanded in Tbx20-overexpressing embryos at the 10-somite stage (A-D), whereas the expression of scl is similar to that of control embryos (E and F). Similarly, myl7 expression is enhanced in Tg(hsp70l:Tbx20) embryos at the 16-somite stage (H). (I and J) Representative confocal projections of myl7:nucGFP hearts from heat-treated non-transgenic (I) and Tg(hsp70l:Tbx20) embryos (J) at 40hpf. (K) Graph of the average number of nucGFP-positive cardiomyocytes in heat-treated non-transgenic (n=8) and Tg(hsp70l:Tbx20) embryos (n=10). Tbx20 overexpression enhanced cardiomyocyte production. (L) Graph of the ratio of BrdU-positive cardiomyocytes to the total number of cardiomyocytes in heat-treated non-transgenic and Tg(hsp70l:Tbx20) embryos (n=7). Overexpression of Tbx20 in early embryos promoted cardiomyocyte proliferation. Error bars indicate standard deviations. Asterisks indicate a significant difference (**p<0.01, ***p<0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP chb2Tg heat shock Prim-25 cardiac muscle cell nucleus IFL
chb2Tg; la2368Tg heat shock Prim-25 cardiac muscle cell nucleus IFL
mef2ca WT heat shock 10-13 somites anterior lateral plate mesoderm heart primordium ISH
la2368Tg heat shock 10-13 somites anterior lateral plate mesoderm heart primordium ISH
myl7 WT heat shock 14-19 somites anterior lateral plate mesoderm heart primordium ISH
14-19 somites cardiac muscle cell ISH
la2368Tg heat shock 14-19 somites anterior lateral plate mesoderm heart primordium ISH
14-19 somites cardiac muscle cell ISH
nkx2.5 WT heat shock 10-13 somites anterior lateral plate mesoderm heart primordium ISH
la2368Tg heat shock 10-13 somites anterior lateral plate mesoderm heart primordium ISH
tal1 WT heat shock 10-13 somites anterior lateral plate mesoderm hematopoietic cell ISH
la2368Tg heat shock 10-13 somites anterior lateral plate mesoderm hematopoietic cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
chb2Tg; la2368Tg heat shock Prim-25 cardiac muscle cell cell population proliferation increased occurrence, abnormal
Prim-25 cardiac muscle cell increased amount, abnormal
la2368Tg heat shock 10-13 somites anterior lateral plate mesoderm heart primordium mef2ca expression increased amount, abnormal
10-13 somites anterior lateral plate mesoderm heart primordium nkx2.5 expression increased amount, abnormal
14-19 somites anterior lateral plate mesoderm heart primordium myl7 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 421(2), Lu, F., Langenbacher, A., Chen, J.N., Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish, 139-148, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.