ZFIN ID: ZDB-FIG-170206-17
Lu et al., 2017 - Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish. Developmental Biology   421(2):139-148 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 10-13 somites to Prim-5

Fig. 2

The LA1110 gene is required for cardiac progenitor formation. (A–J) Dorsal views of nkx2.5 (A and B), mef2ca (C and D) and myl7 (E–J) expression in wild type and LA1110 mutant embryos. In wild type embryos, expression of nkx2.5 (A) and mef2ca (C) are detected in the cardiac progenitors within the ALPM at the 10-somite stage. These cells then differentiate into myl7-expressing cardiomyocytes (E) by the 16-somite stage and migrate toward the midline and fuse to form a cardiac cone (G) which then grows into a linear heart tube by 26 hpf (I). In LA1110 mutant embryos, expression of nkx2.5 (B) and mef2ca (D) is strongly reduced. Even though the expression of myl7 is far lower (F), the myl7-expressing cardiomyocytes in LA1110 mutant embryos migrate to the midline and form the cardiac cone at the 20-somite stage (H) and the linear heart tube at 26 hpf (J).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mef2ca WT control 10-13 somites anterior lateral plate mesoderm heart primordium ISH
tbx20la1110/la1110 control 10-13 somites anterior lateral plate mesoderm heart primordium ISH
myl7 WT control 14-19 somites cardiac muscle cell ISH
20-25 somites cardiac muscle cell ISH
Prim-5 cardiac muscle cell ISH
tbx20la1110/la1110 control 14-19 somites cardiac muscle cell ISH
20-25 somites cardiac muscle cell ISH
Prim-5 cardiac muscle cell ISH
nkx2.5 WT control 10-13 somites anterior lateral plate mesoderm heart primordium ISH
10-13 somites cardiac muscle cell ISH
tbx20la1110/la1110 control 10-13 somites anterior lateral plate mesoderm heart primordium ISH
10-13 somites cardiac muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx20la1110/la1110 control 10-13 somites anterior lateral plate mesoderm heart primordium mef2ca expression decreased amount, abnormal
10-13 somites anterior lateral plate mesoderm heart primordium nkx2.5 expression decreased amount, abnormal
10-13 somites heart primordium hypoplastic, abnormal
14-19 somites cardiac muscle cell decreased amount, abnormal
20-25 somites cardiac muscle cell decreased amount, abnormal
Prim-5 cardiac muscle cell decreased amount, abnormal
Prim-5 heart tube decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 421(2), Lu, F., Langenbacher, A., Chen, J.N., Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish, 139-148, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.