ZFIN ID: ZDB-FIG-170206-20
Lu et al., 2017 - Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish. Developmental Biology   421(2):139-148 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Long-pec

Fig. 5

Tbx20 overexpression rescues the cardiac defects present in tbx20 mutant embryos. (A) Diagram of the inducible activity of the tbx20-GR (glucocorticoid receptor) fusion protein. The addition of DXS (dexamethasone) is required for the translocation of the tbx20-GR protein into the nucleus. (B-D) Dorsal views of myl7 expression at the 16-somite stage in wild type embryos (B), tbx20 mutants (C) and tbx20 mutants injected with tbx20-GR mRNA (D). The cardiomyocyte production defects in tbx20 mutant embryos are substantially rescued by the injection of tbx20-GR mRNA. (E) Schematic representation of the hsp70l:Tbx20 transgene used for tbx20 overexpression. (F-H) Dorsal views of myl7 expression at the 16-somite stage in wild type (F), tbx20 mutant (G) and Tbx20-/-; Tg(hsp70l:Tbx20) embryos (H). (I-K) Frontal views of myl7 expression at 48hpf in wild type (I), tbx20 mutant (J) and Tbx20-/-;Tg(hsp70l:Tbx20) embryos (K). Overexpression of Tbx20 successfully restored cardiomyocyte production in tbx20 mutant embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 WT control 14-19 somites anterior lateral plate mesoderm heart primordium ISH
14-19 somites cardiac muscle cell ISH
Long-pec cardiac muscle cell ISH
Long-pec heart ISH
tbx20la1110/la1110 control 14-19 somites anterior lateral plate mesoderm heart primordium ISH
14-19 somites cardiac muscle cell ISH
Long-pec cardiac muscle cell ISH
Long-pec heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx20la1110/la1110 control 14-19 somites anterior lateral plate mesoderm heart primordium myl7 expression decreased amount, abnormal
Long-pec heart myl7 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 421(2), Lu, F., Langenbacher, A., Chen, J.N., Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish, 139-148, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.