FIGURE

Fig. S3

ID

ZDB-FIG-161122-29

Publication

Katz et al., 2016 - A Nuclear Role for miR-9 and Argonaute Proteins in Balancing Quiescent and Activated Neural Stem Cell States

Other Figures

All Figure Page

Back to All Figure Page

Fig. S3

Validation of efficient Notch blockade and effects of miR-9 knock-down on Notch target genes, related to figure 3

(A) Fluorescent immunostaining of PCNA (green) after 48h of Notch inhibition with LY (bottom panel) or the DMSO control (top panel). Scale bar= 20μm. (B) Percentage of activated NSCs (PCNA+/GFAP+) upon LY vs DMSO treatment. n=3 brains per condition; data are represented as mean ± 95% CI. (**) p<0.01; one-way ANOVA with Bonferroni post-hoc correction. (C-E) ISH using specific probes for notch3(C) and nrarpA (D, E) 2dpi with the miR-9 MO (bottom panels) or control MO (top panels). Scale bars=100μm (C, D) or 40μm (E). (F) Proposed mechanism of action of miR-9 on quiescence, through potentiating Notch activity in NSCs.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.