FIGURE

Fig. S1

ID

ZDB-FIG-161122-27

Publication

Katz et al., 2016 - A Nuclear Role for miR-9 and Argonaute Proteins in Balancing Quiescent and Activated Neural Stem Cell States

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Validation of markers and of the specificity of the LNA miR-9 probe, related to figure 1.

(A) Schematic representation of the germinal zone of the adult zebrafish pallium. Radial glia cells (GFAP+/GS+) lining the ventricle are bona fide NSCs and can be found in two states: activated or quiescent. The activated state is defined by the expression of proliferation markers (MCM2/MCM5/PCNA). Once activated, NSCs give rise to non-glial intermediate precursors which are committed towards neuronal differentiation. (B) Validation of the markers used in this study. All radial glia NSCs co-express GFP driven by the gfap promoter (green, gfap:GFP) and GS (magenta) (top panel). A small fraction of NSCs are found in an activated state in which they coexpress proliferation markers (MCM2, light blue; PCNA, magenta) (bottom panel). Scale bars=50μm (C) ISH of pri-miR-9-2 demonstrating that it is not expressed in the adult telencephalon. Scale Scale bar=100μm. (D) ISH using LNA probes for miR-9-3p (magenta, top panel) and a control sequence (magenta, bottom panel) with immunofluorescence for GS (green) and nuclear DAPI counterstaining (dark blue) demonstrating the specificity of the LNA probes used for mature miR-9-5p detection. In contrast to miR-9- 5p, miR-9-3p expression is much fainter and more diffuse although it is present at very low levels in the nucleus of some ventricular NSCs. (E) ISH using LNA 2'-O-Me probes for miR-9-5p (magenta, top panel) and a control sequence (magenta, bottom panel) with immunofluorescence for GS (green) and nuclear DAPI counterstaining (dark blue) confirming the exclusive expression of miR-9-5p in the ventricular NSCs. Scale bars=20μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.