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Fig. S3
Validation of efficient Notch blockade and effects of miR-9 knock-down on Notch target genes, related to figure 3
(A) Fluorescent immunostaining of PCNA (green) after 48h of Notch inhibition with LY (bottom panel) or the DMSO control (top panel). Scale bar= 20μm. (B) Percentage of activated NSCs (PCNA+/GFAP+) upon LY vs DMSO treatment. n=3 brains per condition; data are represented as mean ± 95% CI. (**) p<0.01; one-way ANOVA with Bonferroni post-hoc correction. (C-E) ISH using specific probes for notch3(C) and nrarpA (D, E) 2dpi with the miR-9 MO (bottom panels) or control MO (top panels). Scale bars=100μm (C, D) or 40μm (E). (F) Proposed mechanism of action of miR-9 on quiescence, through potentiating Notch activity in NSCs.