ZFIN ID: ZDB-FIG-161027-15
Cox et al., 2016 - Selenoprotein H is an essential regulator of redox homeostasis that cooperates with p53 in development and tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America   113(38):E5562-71 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. S5

The seph mutant phenotype is partially mitigated by loss of p53. (A) WISH analysis of elavl3 (neurons) expression and Alcian blue staining in WT, seph-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. (Scale bar: 200 µm.) (B) qPCR expression analysis of the neuron-specific gene elavl3 in WT, tp53-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. n = 3. **P < 0.01. (C) Immunohistochemical detection of PCNA (proliferation) from transverse sections from WT, seph-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. (Scale bar: 50 µm.) (D) Immunohistochemical detection of cleaved caspase-3 (apoptosis) from transverse sections from WT, seph-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. (Scale bar: 50 µm.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
elavl3 AB standard conditions Day 5 neuron ISH
Day 5 whole organism RTPCR
selenohhi2737Tg/hi2737Tg standard conditions Day 5 neuron ISH
Day 5 whole organism RTPCR
tp53zdf1/zdf1; selenohhi2737Tg/hi2737Tg standard conditions Day 5 neuron ISH
Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
selenohhi2737Tg/hi2737Tg standard conditions Day 5 cartilage development process quality, abnormal
Day 5 embryonic viscerocranium morphogenesis process quality, abnormal
Day 5 neuron elavl3 expression decreased amount, abnormal
Day 5 whole organism elavl3 expression decreased amount, abnormal
tp53zdf1/zdf1; selenohhi2737Tg/hi2737Tg standard conditions Day 5 cartilage development process quality, abnormal
Day 5 embryonic viscerocranium morphogenesis process quality, abnormal
Day 5 neuron elavl3 expression decreased amount, abnormal
Day 5 whole organism elavl3 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA