ZFIN ID: ZDB-FIG-161027-11
Cox et al., 2016 - Selenoprotein H is an essential regulator of redox homeostasis that cooperates with p53 in development and tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America   113(38):E5562-71 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Days 7-13

Fig. S1

seph mutant Zebrafish exhibit defects in organ development. (A) Sequence of seph mutant reveals the viral insertion in the 3′ UTR that displaces the SECIS regulatory element that is required for selenoprotein synthesis. (B) WISH analysis of seph expression in WT and seph mutants at 3 dpf. (Scale bar: 200 µm.) (C) Fluorescent imaging of WT and seph mutant larvae on a Tg(gata6:GFP) background at 5 dpf. (Scale bar: 200 µm.) (D) Fluorescent imaging of WT and seph mutant larvae on a Tg(fabp2:dsRed) background at 7 dpf. (Scale bar: 200 µm.) (E) WISH analysis of fabp10a (liver), trypsin (pancreas), and fabp2 (intestine) expression in WT and seph morphants (2 ng, ATG morpholino) at 3 dpf. (Scale bar: 200 µm.) (F) WISH of fabp10a (liver), trypsin (pancreas), and fabp2 (intestine) expression in WT and seph mutants at 3 dpf. (Scale bar: 200 µm.) (G) Morphological assessment illustrating the rescue of the seph mutant phenotype by the injection of SelH-GFP mRNA (GFP-SelHSec38Cys and SelHSec38Cys-GFP constructs) at the one-cell stage. (H) Quantitative determination of the rescue of the seph mutant phenotype by the injection of SelH-GFP mRNA (GFP-SelHSec38Cys and SelHSec38Cys-GFP constructs) at the one-cell stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fabp2 AB control Protruding-mouth intestine ISH
AB + MO1-selenoh standard conditions Protruding-mouth intestine ISH
selenohhi2737Tg/hi2737Tg standard conditions Protruding-mouth intestine ISH
fabp10a AB control Protruding-mouth liver ISH
AB + MO1-selenoh standard conditions Protruding-mouth liver ISH
selenohhi2737Tg/hi2737Tg standard conditions Protruding-mouth liver ISH
prss1 AB control Protruding-mouth pancreas ISH
AB + MO1-selenoh standard conditions Protruding-mouth pancreas ISH
selenohhi2737Tg/hi2737Tg standard conditions Protruding-mouth pancreas ISH
selenoh AB standard conditions Protruding-mouth head ISH
selenohhi2737Tg/hi2737Tg standard conditions Protruding-mouth Not Detected head ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-selenoh standard conditions Protruding-mouth digestive tract development disrupted, abnormal
Protruding-mouth intestine fabp2 expression decreased amount, abnormal
Protruding-mouth liver fabp10a expression decreased amount, abnormal
Protruding-mouth liver development disrupted, abnormal
Protruding-mouth pancreas prss1 expression decreased amount, abnormal
Protruding-mouth pancreas development disrupted, abnormal
selenohhi2737Tg/hi2737Tg standard conditions Protruding-mouth digestive tract development disrupted, abnormal
Protruding-mouth head selenoh expression absent, abnormal
Protruding-mouth intestine fabp2 expression decreased amount, abnormal
Protruding-mouth liver fabp10a expression decreased amount, abnormal
Protruding-mouth liver development disrupted, abnormal
Protruding-mouth pancreas prss1 expression decreased amount, abnormal
Protruding-mouth pancreas development disrupted, abnormal
selenohhi2737Tg/hi2737Tg; ae5Tg standard conditions Day 5 digestive tract development disrupted, abnormal
selenohhi2737Tg/hi2737Tg; zf129Tg standard conditions Days 7-13 intestinal epithelial cell development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA