ZFIN ID: ZDB-FIG-161027-14
Cox et al., 2016 - Selenoprotein H is an essential regulator of redox homeostasis that cooperates with p53 in development and tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America   113(38):E5562-71 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. S4

p53 activation and cell cycle arrest contribute to the SepH-deficient phenotype. (A) Whole-mount immunohistochemical analysis of BrdU incorporation in WT and seph mutants at 5 dpf. Arrows point to clusters of BrdU-positive cells. (Scale bar: 200 µm.) (B) FACS analysis of the cell cycle in WT (blue) and seph mutants (red) at 5 dpf as determined by Hoechst staining (VioFL1). (C) qPCR expression analysis of specific genes associated with p53 activation (rps27l and mdm2) and an inflammatory response (tnfa and il11a) in WT and tp53-/-;seph-/- mutant larvae at 5 dpf. n = 3. **P < 0.01. (D) WISH analysis of fabp10a (liver), trypsin (pancreas), and fabp2 (intestine) expression in WT, seph-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. (Scale bar: 200 µm.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fabp2 AB control Day 5 intestine ISH
selenohhi2737Tg/hi2737Tg standard conditions Day 5 intestine ISH
tp53zdf1/zdf1; selenohhi2737Tg/hi2737Tg standard conditions Day 5 intestine ISH
fabp10a AB control Day 5 liver ISH
selenohhi2737Tg/hi2737Tg standard conditions Day 5 liver ISH
tp53zdf1/zdf1; selenohhi2737Tg/hi2737Tg standard conditions Day 5 liver ISH
prss1 AB control Day 5 pancreas ISH
selenohhi2737Tg/hi2737Tg standard conditions Day 5 pancreas ISH
tp53zdf1/zdf1; selenohhi2737Tg/hi2737Tg standard conditions Day 5 pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
selenohhi2737Tg/hi2737Tg standard conditions Day 5 intestine fabp2 expression decreased amount, abnormal
Day 5 liver fabp10a expression decreased amount, abnormal
Day 5 pancreas prss1 expression decreased amount, abnormal
Day 5 whole organism cell cycle process quality, abnormal
tp53zdf1/zdf1; selenohhi2737Tg/hi2737Tg standard conditions Day 5 intestine fabp2 expression amount, ameliorated
Day 5 liver fabp10a expression amount, ameliorated
Day 5 pancreas prss1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA