ZFIN ID: ZDB-FIG-161027-12
Cox et al., 2016 - Selenoprotein H is an essential regulator of redox homeostasis that cooperates with p53 in development and tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America   113(38):E5562-71 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 3

SepH deficient larvae are prone to oxidative stress. (A) Fluorescent detection of H2O2 (PF2 fluorescence) in WT and seph mutant larvae at 5 dpf. (Scale bar: 200 µm.) (B) Quantification of H2O2 in WT and seph mutant larvae at 5 dpf. n = 5. **P < 0.01. (C) Morphological assessment of WT and seph mutant larvae exposed to menadione (1 µM) from 3 to 5 dpf. (Scale bar: 200 µm.) (D) Quantification of phenotype (unaffected, moderate, or severe) observed following menadione (1 µM) exposure from 3 to 5 dpf. Moderately affected larvae include minor defects, such as edema and tail curling, whereas severely affected larvae include major defects, such as widespread tissue necrosis (loss of transparency).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
selenohhi2737Tg/hi2737Tg standard conditions Day 5 response to oxidative stress process quality, abnormal
selenohhi2737Tg/hi2737Tg chemical treatment: menadione Protruding-mouth - Day 5 post-vent region curved, abnormal
Protruding-mouth - Day 5 trunk curved, abnormal
Protruding-mouth - Day 5 whole organism edematous, abnormal
Protruding-mouth - Day 5 whole organism necrotic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA