ZFIN ID: ZDB-FIG-161027-13
Cox et al., 2016 - Selenoprotein H is an essential regulator of redox homeostasis that cooperates with p53 in development and tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America   113(38):E5562-71 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 5

seph mutants are sensitive to DNA damage. (A) Immunoblot analysis of p53 expression in WT and seph mutant larvae at 5 dpf. (B) Survival of WT and seph mutant larvae 2 dpe to UV irradiation (200 J/m2, 7 dpf). n = 7. ****P < 0.0001. (C) Survival of WT and seph mutant larvae 2 dpe to camptothecin (1 µM). n = 10. ****P < 0.0001. (D) Morphological and histological analysis of WT, seph-/- and tp53-/-;seph-/- mutant larvae at 5 dpf. The yellow dashed region represents the liver. The region of histology showing the larval liver is shown in the zoomed images. [Scale bars: 200 µm (brightfield; BF) and 50 µm (histology; H+E).] (E) Quantitative analysis of liver, pancreas, and gut area in WT, seph-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. n > 8. *P < 0.05; ****P < 0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tp53 Ab11-tp53 AB standard conditions Day 5 whole organism WB
Ab11-tp53 selenohhi2737Tg/hi2737Tg standard conditions Day 5 whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
selenohhi2737Tg/hi2737Tg standard conditions Day 5 eye decreased size, abnormal
Day 5 gut decreased size, abnormal
Day 5 hepatocyte apoptotic, abnormal
Day 5 hepatocyte ballooning, abnormal
Day 5 liver decreased size, abnormal
Day 5 pancreas decreased size, abnormal
Day 5 whole organism tp53 expression increased amount, abnormal
selenohhi2737Tg/hi2737Tg chemical treatment: camptothecin Days 7-13 DNA damage response, detection of DNA damage process quality, abnormal
Days 7-13 whole organism dead, abnormal
Days 7-13 whole organism decreased life span, abnormal
selenohhi2737Tg/hi2737Tg ultraviolet light Days 7-13 DNA damage response, detection of DNA damage process quality, abnormal
Days 7-13 whole organism dead, abnormal
Days 7-13 whole organism decreased life span, abnormal
tp53zdf1/zdf1; selenohhi2737Tg/hi2737Tg standard conditions Day 5 eye size, ameliorated
Day 5 gut size, ameliorated
Day 5 hepatocyte apoptotic process process quality, ameliorated
Day 5 hepatocyte morphology, ameliorated
Day 5 liver size, ameliorated
Day 5 pancreas decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA