ZFIN ID: ZDB-IMAGE-161027-14
Figures for Cox et al., 2016

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Fig. S5

The seph mutant phenotype is partially mitigated by loss of p53. (A) WISH analysis of elavl3 (neurons) expression and Alcian blue staining in WT, seph-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. (Scale bar: 200 µm.) (B) qPCR expression analysis of the neuron-specific gene elavl3 in WT, tp53-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. n = 3. **P < 0.01. (C) Immunohistochemical detection of PCNA (proliferation) from transverse sections from WT, seph-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. (Scale bar: 50 µm.) (D) Immunohistochemical detection of cleaved caspase-3 (apoptosis) from transverse sections from WT, seph-/-, and tp53-/-;seph-/- mutant larvae at 5 dpf. (Scale bar: 50 µm.)

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