FIGURE

Fig. 8

ID

ZDB-FIG-160517-8

Publication

Banote et al., 2016 - β-Amyloid precursor protein-b is essential for Mauthner cell development in the zebrafish in a Notch-dependent manner

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Fig. 8

Maintained identity but changed size of rhombomere 3-5 in Appb hypomorphs. Detection of krox20 (A-B), hoxb1a (C-D) and fgf3 (E-F) mRNA expression by in situ hybridization in the control MO (A, C, E) and appb MO (B, D, F) injected embryos at 18 hpf. The position of the Otic vesicles (ov) is indicated by broken lines. Graph of the anterior to the posterior size of rhombomere 3-5 in control and appb MO injected embryos (G) based on krox20 expression. Cranial motor neurons of the Tg(isl1:GFP) transgenic embryos at 48 hpf (H-I). Anterior (Va) and posterior (Vp) trigeminal nuclei in rhombomere 2 and 3 respectively, facial branchiomotor neurons (VII) in rhombomere 6 and vagal motor neurons (X). Differentiation of these neurons is unaffected in morphant. Dorsal views of the embryos are shown; anterior at the top (A-G); anterior at the left side (H-I). r3-r5, rhombomere 3-5. ****p<0.0001, ns, not significant. Scale bars, 50 µm.

Expression Data

Genes:	egr2b fgf3 GFP hoxb1a
Fish:	WT WT + MO5-appb rw0Tg rw0Tg + MO5-appb
Knockdown Reagent:	MO5-appb
Anatomical Terms:	motor nucleus of vagal nerve rhombomere 2 trigeminal motor nucleus rhombomere 3 rhombomere 3 trigeminal motor nucleus rhombomere 4 rhombomere 5 rhombomere 6 facial nerve motor nucleus
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO5-appb rw0Tg + MO5-appb
Knockdown Reagent:	MO5-appb
Observed In:	facial nerve motor nucleus central nervous system neuron differentiation rhombomere 3 rhombomere 3 morphogenesis rhombomere 4 rhombomere 4 morphogenesis
Stage Range:	14-19 somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 413(1), Banote, R.K., Edling, M., Eliassen, F., Kettunen, P., Zetterberg, H., Abramsson, A., β-Amyloid precursor protein-b is essential for Mauthner cell development in the zebrafish in a Notch-dependent manner, 26-38, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.