ZFIN ID: ZDB-FIG-160517-7
Banote et al., 2016 - β-Amyloid precursor protein-b is essential for Mauthner cell development in the zebrafish in a Notch-dependent manner. Developmental Biology   413(1):26-38 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 7

DAPT inhibition of γ-secretase and reduced Notch1a rescues Mauthner cell number in Appb morphants. RMO44 immunostaining of embryos at 48 hpf (A-D), anterior at the top. DAPT-treated embryo has supernumerary M-cell (A). DAPT treatment of appb MO injected embryos balances the number of M-cell (B). Embryo co-injected with control MO and notch1a MO has supernumerary M-cells (C). Co-injection of appb MO and notch1a MO rescues the number of M-cell (D). Bar graph displays the quantification of M-cell phenotype in DAPT treated (E) and notch1a injected embryos (F). n, number of embryos. Scale bar, 50 µm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-neurofilament-m IHC WT chemical treatment by environment: DAPT Long-pec Mauthner neuron axon
IHC Long-pec Mauthner neuron cell body
IHC WT + MO3-notch1a + MO4-notch1a control Long-pec Mauthner neuron axon
IHC Long-pec Mauthner neuron cell body
IHC WT + MO3-notch1a + MO4-notch1a + MO5-appb standard conditions Long-pec Mauthner neuron axon
IHC Long-pec Mauthner neuron cell body
IHC WT + MO5-appb chemical treatment by environment: DAPT Long-pec Mauthner neuron axon
IHC Long-pec Mauthner neuron cell body
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: DAPT Long-pec hindbrain has extra parts of type Mauthner neuron, abnormal
WT + MO3-notch1a + MO4-notch1a control Long-pec hindbrain has extra parts of type Mauthner neuron, abnormal
WT + MO3-notch1a + MO4-notch1a + MO5-appb standard conditions Long-pec hindbrain has number of Mauthner neuron, ameliorated
WT + MO5-appb standard conditions Long-pec hindbrain lacks parts or has fewer parts of type Mauthner neuron, abnormal
WT + MO5-appb chemical treatment by environment: DAPT Long-pec hindbrain has number of Mauthner neuron, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 413(1), Banote, R.K., Edling, M., Eliassen, F., Kettunen, P., Zetterberg, H., Abramsson, A., β-Amyloid precursor protein-b is essential for Mauthner cell development in the zebrafish in a Notch-dependent manner, 26-38, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.