|ZFIN ID: ZDB-FIG-160517-7|
DAPT inhibition of γ-secretase and reduced Notch1a rescues Mauthner cell number in Appb morphants. RMO44 immunostaining of embryos at 48 hpf (A-D), anterior at the top. DAPT-treated embryo has supernumerary M-cell (A). DAPT treatment of appb MO injected embryos balances the number of M-cell (B). Embryo co-injected with control MO and notch1a MO has supernumerary M-cells (C). Co-injection of appb MO and notch1a MO rescues the number of M-cell (D). Bar graph displays the quantification of M-cell phenotype in DAPT treated (E) and notch1a injected embryos (F). n, number of embryos. Scale bar, 50 µm.
|Ab1-neurofilament-m||IHC||WT||chemical treatment by environment: DAPT||Long-pec|
|IHC||WT + MO3-notch1a + MO4-notch1a||control||Long-pec|
|IHC||WT + MO3-notch1a + MO4-notch1a + MO5-appb||standard conditions||Long-pec|
|IHC||WT + MO5-appb||chemical treatment by environment: DAPT||Long-pec|
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
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Reprinted from Developmental Biology, 413(1), Banote, R.K., Edling, M., Eliassen, F., Kettunen, P., Zetterberg, H., Abramsson, A., β-Amyloid precursor protein-b is essential for Mauthner cell development in the zebrafish in a Notch-dependent manner, 26-38, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.