ZFIN Image: Banote et al., 2016, Fig. 8

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Figure Caption

Fig. 8

Maintained identity but changed size of rhombomere 3-5 in Appb hypomorphs. Detection of krox20 (A-B), hoxb1a (C-D) and fgf3 (E-F) mRNA expression by in situ hybridization in the control MO (A, C, E) and appb MO (B, D, F) injected embryos at 18 hpf. The position of the Otic vesicles (ov) is indicated by broken lines. Graph of the anterior to the posterior size of rhombomere 3-5 in control and appb MO injected embryos (G) based on krox20 expression. Cranial motor neurons of the Tg(isl1:GFP) transgenic embryos at 48 hpf (H-I). Anterior (Va) and posterior (Vp) trigeminal nuclei in rhombomere 2 and 3 respectively, facial branchiomotor neurons (VII) in rhombomere 6 and vagal motor neurons (X). Differentiation of these neurons is unaffected in morphant. Dorsal views of the embryos are shown; anterior at the top (A-G); anterior at the left side (H-I). r3-r5, rhombomere 3-5. ****p<0.0001, ns, not significant. Scale bars, 50 µm.

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Acknowledgments

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Reprinted from Developmental Biology, 413(1), Banote, R.K., Edling, M., Eliassen, F., Kettunen, P., Zetterberg, H., Abramsson, A., β-Amyloid precursor protein-b is essential for Mauthner cell development in the zebrafish in a Notch-dependent manner, 26-38, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.