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ZIRC
ZFIN ID: ZDB-FIG-160226-72
Gonsar et al., 2016 - Temporal and spatial requirements for Nodal-induced anterior mesendoderm and mesoderm in anterior neurulation. Genesis (New York, N.Y. : 2000)   54(1):3-18 Full text @ Genesis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 26+ somites to Prim-15

Fig. 9

Closed neural tubes and normal mesendoderm/mesoderm development in cyc and cas mutants. Embryos were coassayed for otx5 expression in the pineal precursors and a combination of the following mesendodermal/mesodermal markers: ctsl1b in the hatching glands, pax 2.1, col9a2 in cephalic paraxial mesoderm, and shh (for cas mutant) or col2a1 in the notochord (WT embryo and cyc mutant). Embryos in the first three rows are at ~26 somite stage and those in the last row are 30 hpf. Images in the same column and of the same age are different views of the same embryo. White arrowheads point to pineal, white arrows to hatching glands. Scale bars: 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a WT standard conditions 26+ somites notochord ISH
ndr2m294/m294 standard conditions 26+ somites notochord ISH
col9a2 WT standard conditions Prim-15 head paraxial mesoderm ISH
ndr2m294/m294 standard conditions Prim-15 head paraxial mesoderm ISH
sox32ta56/ta56 standard conditions Prim-15 head paraxial mesoderm ISH
ctslb WT standard conditions 26+ somites hatching gland cell ISH
ndr2m294/m294 standard conditions 26+ somites hatching gland cell ISH
sox32ta56/ta56 standard conditions 26+ somites hatching gland cell ISH
otx5 WT standard conditions 26+ somites pineal complex ISH
ndr2m294/m294 standard conditions 26+ somites pineal complex ISH
sox32ta56/ta56 standard conditions 26+ somites pineal complex ISH
shha sox32ta56/ta56 standard conditions 26+ somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Genesis (New York, N.Y. : 2000) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genesis