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ZFIN ID: ZDB-FIG-160226-69
Gonsar et al., 2016 - Temporal and spatial requirements for Nodal-induced anterior mesendoderm and mesoderm in anterior neurulation. Genesis (New York, N.Y. : 2000)   54(1):3-18 Full text @ Genesis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 6

Positive correlation between neural tube closure and the presence of prechordal plate-derived hatching glands and axial mesoderm-derived anterior notochord. Embryos were raised to ~1 dpf and processed in tandem for otx5 in the pineal anlage, col2a1 in the notochord, and cstl1b in the hatching gland cells (hgg). (A) In WT embryos, the pineal organ is oval indicating a closed neural tube, the notochord spans the complete anterior–posterior extent of the trunk, and the hatching glands make a “necklace” around the anterior of the head. sqt embryos have a range of neural tube, notochord, and hgg defects as indicated. Note that, at this stage, col2a1 is expressed in three adjacent lines of cells, the floor plate (dorsal to the notochord), the hypochord (ventral to the notochord), and the notochord. The gaps in staining in sqt embryo 1 correspond to loss of all three tissues. The narrow region of staining in sqt embryo 3 just above the hind yolk corresponds to a place where the floor plate and hypochord cells persist, but the wider notochord cells are missing. The neural tube was scored as open if the pineal organ was at least twice as wide along its left–right axis (brackets) compared to its anterior–posterior axis. Images in the same row are different views of the same embryo. First column: dorsal views with anterior to the top. Second column: lateral views with anterior to the left and dorsal to the top. Third column: frontal views with anterior to the top. White arrows point to hatching glands. Scale bars: 100 µm. (B, C) Complete set of quantitative data.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a WT standard conditions Prim-5 floor plate ISH
Prim-5 hypochord ISH
Prim-5 notochord ISH
ndr1cz35/cz35 standard conditions Prim-5 floor plate ISH
Prim-5 hypochord ISH
Prim-5 notochord ISH
ctslb WT standard conditions Prim-5 hatching gland cell ISH
ndr1cz35/cz35 standard conditions Prim-5 hatching gland cell ISH
otx5 WT standard conditions Prim-5 pineal complex ISH
ndr1cz35/cz35 standard conditions Prim-5 pineal complex ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ndr1cz35/cz35 standard conditions Prim-5 eye fused with eye, abnormal
Prim-5 floor plate col2a1a expression decreased amount, abnormal
Prim-5 hatching gland cell ctslb expression decreased amount, abnormal
Prim-5 hatching gland cell decreased amount, abnormal
Prim-5 hypochord col2a1a expression decreased amount, abnormal
Prim-5 neural tube open, abnormal
Prim-5 notochord aplastic/hypoplastic, abnormal
Prim-5 notochord col2a1a expression decreased amount, abnormal
Prim-5 pineal complex elongated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Genesis (New York, N.Y. : 2000) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genesis