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ZFIN ID: ZDB-FIG-160226-59
Kao et al., 2015 - Pbx4 is Required for the Temporal Onset of Zebrafish Myocardial Differentiation. Journal of developmental biology   3:93-111 Full text @ J Dev Biol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 10-13 somites to Prim-5

Fig. 2

Delayed onset of cardiomyocyte differentiation genes in pbx4 mutant embryos. (AT, VW) RNA in situ expression of cardiomyocyte differentiation genes (AD) tnnt2a, (EF, IJ, OP) myl7, (GH, KL, QR) vmhc, (MN, ST) myh6, and (VW) nppa in (A,C,E,G,I,K,M,O,Q,S,V) control and (B,D,F,H,J,L,N,P,R,T,W) pbx4b557-/- embryos. Developmental stages are indicated. Embryos are shown in dorsal view, anterior towards the left. (AH) Expression of the Pbx-dependent gene krox20, marking rhombomeres 3 and 5 (r3, r5 in A) in the hindbrain, was included to distinguish between control and pbx4b557-/- mutants [12,19]. Expression of myod, which is expressed in a stripe in each somite plus two additional pre-somitic stripes [38], was included for somite staging. Arrows indicate ALPM expression domains of cardiomyocyte differentiation genes. (A,B) At 12 s, tnnt2a is absent or reduced in pbx4b557-/- embryos (n = 8; 6/8 absent, 2/8 reduced) compared to controls (n = 30; all similar). (C,D) at 16 s, tnnt2a is reduced in pbx4b557-/- embryos (n = 7) compared to controls (n = 28). (E,F) at 16 s, myl7 is absent or reduced in pbx4b557-/- embryos (n = 9; 3/9 absent, 6/9 reduced) compared to controls (n = 22). (G,H) at 16 s, vmhc is absent or reduced in pbx4b557-/- embryos (n = 6; 1/6 absent, 5/6 reduced) compared to controls (n = 10). (IT) At 21 s and 27 s, myl7, vmhc, and myh6 show delayed fusion and abnormal patterning of expression domains in pbx4b557-/- embryos compared to controls. For (IN), numbers of affected embryos are provided in the text. For (OT), numbers of affected embryos are graphed in (U). Arrows indicate expression domains of cardiomyocyte differentiation genes. (U) Graph displaying percentage of embryos at 27 s with either normally patterned (blue), dysmorphic or unfused (green), or anteriorly fused (purple) cardiac primordia. (VW) At 27 hpf, nppa is reduced in pbx4b557-/- embryos (n = 4, all similar reduced expression) compared to controls (n = 24). Arrows indicate myocardial expression domains.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b AB control 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
14-19 somites rhombomere 3 ISH
14-19 somites rhombomere 5 ISH
pbx4b557/b557(AB) standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
14-19 somites rhombomere 3 ISH
14-19 somites rhombomere 5 ISH
myh6 AB control 20-25 somites cardiac muscle cell ISH
26+ somites cardiac muscle cell ISH
pbx4b557/b557(AB) standard conditions 20-25 somites cardiac muscle cell ISH
26+ somites cardiac muscle cell ISH
myh7 AB control 14-19 somites anterior lateral plate mesoderm ISH
14-19 somites anterior lateral plate mesoderm cardiac muscle cell ISH
20-25 somites cardiac muscle cell ISH
26+ somites cardiac muscle cell ISH
pbx4b557/b557(AB) standard conditions 14-19 somites anterior lateral plate mesoderm ISH
14-19 somites anterior lateral plate mesoderm cardiac muscle cell ISH
20-25 somites cardiac muscle cell ISH
26+ somites cardiac muscle cell ISH
myl7 AB control 14-19 somites anterior lateral plate mesoderm ISH
14-19 somites anterior lateral plate mesoderm cardiac muscle cell ISH
20-25 somites cardiac muscle cell ISH
26+ somites cardiac muscle cell ISH
pbx4b557/b557(AB) standard conditions 14-19 somites anterior lateral plate mesoderm ISH
14-19 somites anterior lateral plate mesoderm cardiac muscle cell ISH
20-25 somites cardiac muscle cell ISH
26+ somites cardiac muscle cell ISH
myod1 AB control 10-13 somites somite ISH
14-19 somites somite ISH
pbx4b557/b557(AB) standard conditions 10-13 somites somite ISH
14-19 somites somite ISH
nppa AB control Prim-5 myocardium ISH
pbx4b557/b557(AB) standard conditions Prim-5 myocardium ISH
tnnt2a AB control 10-13 somites anterior lateral plate mesoderm ISH
10-13 somites anterior lateral plate mesoderm cardiac muscle cell ISH
14-19 somites anterior lateral plate mesoderm ISH
14-19 somites anterior lateral plate mesoderm cardiac muscle cell ISH
pbx4b557/b557(AB) standard conditions 10-13 somites anterior lateral plate mesoderm ISH
10-13 somites anterior lateral plate mesoderm cardiac muscle cell ISH
14-19 somites anterior lateral plate mesoderm ISH
14-19 somites anterior lateral plate mesoderm cardiac muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pbx4b557/b557(AB) standard conditions 10-13 somites anterior lateral plate mesoderm tnnt2a expression decreased amount, abnormal
10-13 somites anterior lateral plate mesoderm cardiac muscle cell tnnt2a expression decreased amount, abnormal
14-19 somites anterior lateral plate mesoderm tnnt2a expression decreased amount, abnormal
14-19 somites anterior lateral plate mesoderm myl7 expression decreased amount, abnormal
14-19 somites anterior lateral plate mesoderm myh7 expression decreased amount, abnormal
14-19 somites anterior lateral plate mesoderm cardiac muscle cell tnnt2a expression decreased amount, abnormal
14-19 somites anterior lateral plate mesoderm cardiac muscle cell myh7 expression decreased amount, abnormal
14-19 somites anterior lateral plate mesoderm cardiac muscle cell myl7 expression decreased amount, abnormal
20-25 somites cardiac muscle cell myh6 expression decreased amount, abnormal
20-25 somites cardioblast migration to the midline involved in heart rudiment formation decreased occurrence, abnormal
26+ somites cardiac muscle cell myh6 expression decreased amount, abnormal
26+ somites cardioblast migration to the midline involved in heart rudiment formation decreased occurrence, abnormal
Prim-5 myocardium nppa expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J Dev Biol