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ZFIN ID: ZDB-FIG-160226-62
Kao et al., 2015 - Pbx4 is Required for the Temporal Onset of Zebrafish Myocardial Differentiation. Journal of developmental biology   3:93-111 Full text @ J Dev Biol
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec

Fig. 5

Analysis of Pbx expression during zebrafish cardiac development. (AG) Pbx immunostaining at (AB) 10 s (14 hpf), (CF) 21 s (19.5 hpf), and (GH) 48 hpf. (AB) Pbx expression (magenta) in wild type 10 s embryo co-stained with Hoechst to label nuclei (green). (A) Dorsal view, anterior to the left. The line in (A), approximately at the level of hindbrain rhombomere 3, shows where we took an optical cross-section view for (B). Arrows in (B) show Pbx expression in ALPM cells next to the neural tube (nt in A,B). (C–F) Pbx expression (magenta) in Tg(myl7:EGFP)twu34 (green) 21 s (CD) control embryo and (EF) pbx4-/- embryo, at low (C,E) and high (D,F) magnification. Arrow in (D) points to cells co-expressing myl7:EGFP and Pbx. Dorsal view, anterior to the left. (G) Pbx expression (magenta) in Tg(myl7:EGFP)twu34 (green) 48 hpf embryo. Ventral view, anterior to the top. V, ventricle. A, atrium. (HI) Pbx expression (magenta) in wild-type 48 hpf embryo co-stained for proepicardial pan-cytokeratin expression (green) and myocardial cells (MF20, red), at low (H) and high (I) magnification. V, ventricle. Arrows in (H,I) point to two pan-cytokeratin-expressing cells that do not express Pbx, compared to neighboring cardiomyocytes and pan-cytokeratin/Pbx co-expressing cells (arrowheads, I). Scale bar for (A): 100 µm. Scale bar for (BD): 50 µm. Scale bar for (H): 10 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg control 20-25 somites cardiac muscle cell IHC
Long-pec cardiac muscle cell IHC
twu34Tg ; pbx4b557/b557 control 20-25 somites cardiac muscle cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC AB control Long-pec cardiac ventricle
Phenotype Details No data available
ZFIN wishes to thank the journal Journal of developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J Dev Biol