FIGURE

Fig. 3

ID

ZDB-FIG-160203-2

Publication

Okuda et al., 2015 - A zebrafish model of inflammatory lymphangiogenesis

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Fig. 3

Zebrafish macrophages and neutrophils express lymphatic growth factors. (A) RT-PCR of vegfaa, vegfc, vegfd, csf1ra, mpx, cdx1b, and ef1-a from RNA isolated from untreated and TNBS-treated 7dpf zebrafish macrophage-lineage cells. (B) qPCR analysis of vegfc mRNA in TNBS-treated 7dpf zebrafish macrophages relative to untreated 7dpf zebrafish macrophages (n=3). (C) RT-PCR of vegfaa, vegfc, vegfd, csf1ra, mpx, cdx1b, and ef1-a from RNA isolated from untreated and TNBS-treated 7dpf zebrafish neutrophils. The colours in the gel images are inverted and the numbers in brackets indicate reproducibility over three experiments. Error bars, ±s.d. n.s. P>0.05 by unpaired t-test.

Expression Data

Genes:	cdx1b csf1ra mpx vegfaa vegfc vegfd
Fish:	gl22Tg nz117Tg
Condition:	chemical treatment: 2,4,6-trinitrobenzenesulfonic acid
Anatomical Terms:	macrophage neutrophil
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz117Tg
Condition:	chemical treatment: 2,4,6-trinitrobenzenesulfonic acid
Observed In:	neutrophil
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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