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ZIRC
ZFIN ID: ZDB-FIG-160203-2
Okuda et al., 2015 - A zebrafish model of inflammatory lymphangiogenesis. Biology Open   4(10):1270-80 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. 3

Zebrafish macrophages and neutrophils express lymphatic growth factors. (A) RT-PCR of vegfaa, vegfc, vegfd, csf1ra, mpx, cdx1b, and ef1-a from RNA isolated from untreated and TNBS-treated 7dpf zebrafish macrophage-lineage cells. (B) qPCR analysis of vegfc mRNA in TNBS-treated 7dpf zebrafish macrophages relative to untreated 7dpf zebrafish macrophages (n=3). (C) RT-PCR of vegfaa, vegfc, vegfd, csf1ra, mpx, cdx1b, and ef1-a from RNA isolated from untreated and TNBS-treated 7dpf zebrafish neutrophils. The colours in the gel images are inverted and the numbers in brackets indicate reproducibility over three experiments. Error bars, ±s.d. n.s. P>0.05 by unpaired t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdx1b gl22Tg control Days 7-13 Not Detected macrophage RTPCR
gl22Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 Not Detected macrophage RTPCR
nz117Tg control Days 7-13 Not Detected neutrophil RTPCR
nz117Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 Not Detected neutrophil RTPCR
csf1ra gl22Tg control Days 7-13 macrophage RTPCR
gl22Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 macrophage RTPCR
nz117Tg control Days 7-13 neutrophil RTPCR
nz117Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 neutrophil RTPCR
mpx gl22Tg control Days 7-13 macrophage RTPCR
gl22Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 macrophage RTPCR
nz117Tg control Days 7-13 neutrophil RTPCR
nz117Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 neutrophil RTPCR
vegfaa gl22Tg control Days 7-13 macrophage RTPCR
gl22Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 macrophage RTPCR
nz117Tg control Days 7-13 neutrophil RTPCR
nz117Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 neutrophil RTPCR
vegfc gl22Tg control Days 7-13 macrophage RTPCR
gl22Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 macrophage RTPCR
nz117Tg control Days 7-13 neutrophil RTPCR
nz117Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 neutrophil RTPCR
vegfd gl22Tg control Days 7-13 macrophage RTPCR
gl22Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 macrophage RTPCR
nz117Tg control Days 7-13 neutrophil RTPCR
nz117Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 neutrophil RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz117Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 neutrophil vegfc expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open