ZFIN ID: ZDB-FIG-151125-22
Okuda et al., 2015 - A zebrafish model of inflammatory lymphangiogenesis. Biology Open   4(10):1270-80 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Days 7-13

Fig. 5

Macrophages are required for intestinal inflammatory lymphangiogenesis. (A) Schema outlining metronidazole and TNBS treatments. (B) Lateral image of the intestine in 7dpf lyve1:DsRed2; kdrl:EGFP larvae exposed to TNBS+2.5mM metronidazole (Met). (C,D), Lateral image of the intestine in 7dpf lyve1:DsRed2;kdrl:EGFP;mpeg1:Gal4FF;UAS:nfsB:mCherry larvae exposed to TNBS (C) and TNBS+2.5mM metronidazole (D). (E,F), Lateral image of the intestine in 7dpf lyve1:DsRed2;kdrl:EGFP;mpx:KalTA4;UAS:nfsB:mCherry larvae exposed to TNBS (E) and TNBS+2.5mM metronidazole (F). (B2-F2) shows expression in the DsRed/mCherry channel only. Asterisks indicate intestinal lymphatic sprouts (ILSs). (G,H) FACS analysis of mCherry-positive cell counts from TNBS-treated (control) and TNBS+2.5mM metronidazole-treated 3 and 7dpf mpeg1:Gal4FF;UAS:nfsB:mCherry larvae (G) or mpx:KalTA4;UAS:nfsB:mCherry larvae (H) (ne3, each replicate contains 30–80 larvae). (I,J) Quantification of ILS number (I) and total ILS length (J) (ne17). Error bars, ±s.d. n.s. P>0.05, *P<0.05, **P<0.01, ***P<0.001, by unpaired t-test (G,H) or by one-way ANOVA with Tukey′s multiple comparison test (I,J). TD, thoracic duct. Scale bar: 100µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed c264Tg; gl25Tg; nz101Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network IFL
Days 7-13 intestinal lymphatic network lymphangiogenic sprout IFL
Days 7-13 lymphatic system IFL
Days 7-13 thoracic duct IFL
c264Tg; gl25Tg; nz101Tg; s843Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network IFL
Days 7-13 intestinal lymphatic network lymphangiogenic sprout IFL
Days 7-13 lymphatic system IFL
Days 7-13 thoracic duct IFL
c264Tg; gl28Tg; nz150Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network IFL
Days 7-13 intestinal lymphatic network lymphangiogenic sprout IFL
Days 7-13 lymphatic system IFL
Days 7-13 thoracic duct IFL
c264Tg; gl28Tg; nz150Tg; s843Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network IFL
Days 7-13 intestinal lymphatic network lymphangiogenic sprout IFL
Days 7-13 lymphatic system IFL
Days 7-13 thoracic duct IFL
nz101Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network IFL
Days 7-13 intestinal lymphatic network lymphangiogenic sprout IFL
Days 7-13 lymphatic system IFL
Days 7-13 thoracic duct IFL
EGFP c264Tg; gl25Tg; nz101Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 blood vasculature IFL
Days 7-13 intestine blood vasculature IFL
c264Tg; gl25Tg; nz101Tg; s843Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 blood vasculature IFL
Days 7-13 intestine blood vasculature IFL
c264Tg; gl28Tg; nz150Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 blood vasculature IFL
Days 7-13 intestine blood vasculature IFL
c264Tg; gl28Tg; nz150Tg; s843Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 blood vasculature IFL
Days 7-13 intestine blood vasculature IFL
nz101Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 blood vasculature IFL
Days 7-13 intestine blood vasculature IFL
mCherry c264Tg; gl25Tg; nz101Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 macrophage IFL
c264Tg; gl25Tg; nz101Tg; s843Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 macrophage IFL
c264Tg; gl28Tg; nz150Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 neutrophil IFL
c264Tg; gl28Tg; nz150Tg; s843Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
c264Tg; gl25Tg; nz101Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network lymphangiogenesis process quality, ameliorated
Days 7-13 intestinal lymphatic network lymphangiogenic sprout amount, ameliorated
Days 7-13 macrophage decreased amount, abnormal
c264Tg; gl25Tg; nz101Tg; s843Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network lymphangiogenesis increased process quality, abnormal
Days 7-13 intestinal lymphatic network lymphangiogenic sprout increased amount, abnormal
c264Tg; gl28Tg; nz150Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network lymphangiogenesis increased process quality, abnormal
Days 7-13 intestinal lymphatic network lymphangiogenic sprout increased amount, abnormal
Days 7-13 neutrophil decreased amount, abnormal
c264Tg; gl28Tg; nz150Tg; s843Tg chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network lymphangiogenesis increased process quality, abnormal
Days 7-13 intestinal lymphatic network lymphangiogenic sprout increased amount, abnormal
nz101Tg; s843Tg chemical treatment: metronidazole, chemical treatment: 2,4,6-trinitrobenzenesulfonic acid Days 7-13 intestinal lymphatic network lymphangiogenesis increased process quality, abnormal
Days 7-13 intestinal lymphatic network lymphangiogenic sprout increased amount, abnormal
Acknowledgments:
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