FIGURE

Fig. 3

ID

ZDB-FIG-151214-10

Publication

Gokey et al., 2015 - The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish

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Fig. 3

Depletion of maternal and zygotic Atp6ap1b disrupted LR patterning. (A–C) RNA in situ hybridizations of the cardiac-specific cmlc2 marker were used to assess heart looping (arrow) at 2 dpf. v=ventricle; a=atrium. Normal rightward looping (A) was observed in wild-type embryos, whereas heart looping often failed (B) or was reversed (C) in embryos injected with two different translation-blocking MOs. (D) Quantification of heart looping phenotypes in atp6ap1b MO embryos. MO-induced heart looping defects were partially rescued by co-injection with atp6ap1b mRNA. (E–H) RNA in situ hybridization analysis of spaw expression (arrow) at 18 somite stage that is left-sided in controls (E), but reversed (F), bilateral (G) or absent (H) in many atp6ap1b MO embryos (I).

Expression Data

Genes:	myl7 spaw
Fish:	WT WT + MO1-atp6ap1b WT + MO2-atp6ap1b
Knockdown Reagents:	MO1-atp6ap1b MO2-atp6ap1b
Anatomical Terms:	heart mesoderm mesoderm left side mesoderm right side
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-atp6ap1b WT + MO2-atp6ap1b
Knockdown Reagents:	MO1-atp6ap1b MO2-atp6ap1b
Observed In:	determination of left/right asymmetry in lateral mesoderm heart looping mesoderm mesoderm right side
Stage Range:	14-19 somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 407(1), Gokey, J.J., Dasgupta, A., Amack, J.D., The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish, 115-30, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.