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Fig. S8

ID

ZDB-FIG-151214-20

Publication

Gokey et al., 2015 - The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish

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Fig. S8

Loss of Atp6ap1b alters subcellular localization of Atp6v1a. (A) In enveloping layer (EVL) cells, Atp6v1a puncta were found in the cytoplasm and some plasma membranes (arrows) in control embryos during epiboly. (B) Plasma membrane localization was reduced in Atp6ap1b depleted embryos. (C) A plasma membrane-to-cytoplasm ratio of Atp6v1a in EVL. (D) Atp6v1a staining in 3 dpf neuromasts localized basally in wild-type hair cells counter stained with phalloidin to detect actin-rich stereocilia. (E) Basal Atp6v1a localization was disrupted atp6ap1ba82/a82 mutants. (F) Basal-to-apical ratio of of Atp6v1a fluorescence in hair cells.

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Acknowledgments

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Reprinted from Developmental Biology, 407(1), Gokey, J.J., Dasgupta, A., Amack, J.D., The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish, 115-30, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.