FIGURE

Fig. 4

ID

ZDB-FIG-151214-11

Publication

Gokey et al., 2015 - The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Inhibiting V-ATPase function disrupted cardiac left–right asymmetry, KV organ size and KV cilia formation. (A) Concanamycin A treatments between the 50% epiboly stage and the 6 somite stage or MO depletion of Atp6v1f altered heart looping as compared to controls. Heart defects in atp6v1f MO embryos were significantly rescued by atp6v1f mRNA. (B–D) Immunofluorescent staining of KV using aPKC and acetylated-Tubulin markers. (E–G) Quantification of KV cilia length (E), cilia number (F), and KV size (G) in DMSO control (n=17) embryos, concanamycin A treated (n=26) embryos and atp6v1f MO injected (n=22) embryos. Scale bars=5 µm.

Expression Data

Antibodies:	Ab1-prkcz Ab1-tuba
Fish:	WT WT + MO1-atp6v1f
Condition:	chemical treatment: concanamycin A
Knockdown Reagent:	MO1-atp6v1f
Anatomical Terms:	Kupffer's vesicle apical plasma membrane Kupffer's vesicle cilium
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-atp6v1f
Condition:	chemical treatment: concanamycin A
Knockdown Reagent:	MO1-atp6v1f
Observed In:	heart looping Kupffer's vesicle Kupffer's vesicle cilium
Stage Range:	5-9 somites to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 407(1), Gokey, J.J., Dasgupta, A., Amack, J.D., The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish, 115-30, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.