ZFIN ID: ZDB-FIG-150826-14
Auer et al., 2015 - Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLIFE   4 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 6 Expression of the neurotrophic factor neurotrophin 3 in visually impaired mutants.

(A) Relative expression levels of bdnf, ntf3, ntf4, ntf7, and ngf in 4 dpf old wild-type and kif5aa mutant embryos. ntf3 is upregulated to 160% of wild-type expression levels (p < 0.05) while all other neurotrophic factors show the same expression levels between wild-type and mutant embryos. (B) Transversal sections through the tectum after in situ hybridization with an ntf3 specific antisense probe detect higher expression levels of ntf3 in tecta of kif5aa. Scale bars = 25 ┬Ám. PC = pigment cell, NP = neuropil, PVN = periventricular neurons. Asterisk = strong ntf3 signal in the otic vesicle. (C) Confirmation of Ntf3 overexpression by Western blotting in 4 dpf old embryos. To show the specificity of the antibody, we generated a Ntf3 overexpression construct (UAS:ntf3-E2A-RFP). The two visually impaired mutant lines lakritz and blumenkohl also show a substantial upregulation of ntf3 expression levels. (D) Quantification of Ntf3 protein expression levels based on Western blotting data in wild-type and visually impaired mutant embryos. All three mutant lines show a substantial upregulation of Ntf3 protein levels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bdnf WT standard conditions Day 4 whole organism RTPCR
kif5aaic3023/ic3023 standard conditions Day 4 whole organism RTPCR
kif5aaic3024/ic3024 standard conditions Day 4 whole organism RTPCR
ngfa WT standard conditions Day 4 whole organism RTPCR
kif5aaic3023/ic3023 standard conditions Day 4 whole organism RTPCR
kif5aaic3024/ic3024 standard conditions Day 4 whole organism RTPCR
ngfb WT standard conditions Day 4 whole organism RTPCR
kif5aaic3023/ic3023 standard conditions Day 4 whole organism RTPCR
kif5aaic3024/ic3024 standard conditions Day 4 whole organism RTPCR
ntf3 WT standard conditions Day 4 optic tectum ISH
Day 4 whole organism RTPCR
Ab1-ntf3 Day 4 whole organism WB
Ab1-ntf3 atoh7th241/th241 standard conditions Day 4 whole organism WB
kif5aaic3023/ic3023 standard conditions Day 4 optic tectum ISH
Day 4 whole organism RTPCR
Ab1-ntf3 Day 4 whole organism WB
kif5aaic3024/ic3024 standard conditions Day 4 optic tectum ISH
Day 4 whole organism RTPCR
Ab1-ntf3 Day 4 whole organism WB
Ab1-ntf3 slc17a6btc257z/tc257z standard conditions Day 4 whole organism WB
ntf4 WT standard conditions Day 4 whole organism RTPCR
kif5aaic3023/ic3023 standard conditions Day 4 whole organism RTPCR
kif5aaic3024/ic3024 standard conditions Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
atoh7th241/th241 standard conditions Day 4 whole organism ntf3 expression increased amount, abnormal
kif5aaic3023/ic3023 standard conditions Day 4 optic tectum ntf3 expression increased amount, abnormal
Day 4 optic tectum ntf3 expression increased distribution, abnormal
Day 4 whole organism ntf3 expression increased amount, abnormal
Day 4 whole organism ntf3 expression increased amount, abnormal
kif5aaic3024/ic3024 standard conditions Day 4 optic tectum ntf3 expression increased amount, abnormal
Day 4 optic tectum ntf3 expression increased distribution, abnormal
Day 4 whole organism ntf3 expression increased amount, abnormal
Day 4 whole organism ntf3 expression increased amount, abnormal
slc17a6btc257z/tc257z standard conditions Day 4 whole organism ntf3 expression increased amount, abnormal
Acknowledgments:
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