PUBLICATION

Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3

Authors
Auer, T.O., Xiao, T., Bercier, V., Gebhardt, C., Duroure, K., Concordet, J.P., Wyart, C., Suster, M., Kawakami, K., Wittbrodt, J., Baier, H., Del Bene, F.
ID
ZDB-PUB-150616-2
Date
2015
Source
eLIFE   4: (Journal)
Registered Authors
Auer, Thomas, Baier, Herwig, Bercier, Valérie, Del Bene, Filippo, Duroure, Karine, Gebhardt, Christoph, Kawakami, Koichi, Suster, Maximiliano, Wittbrodt, Jochen, Wyart, Claire, Xiao, Tong
Keywords
axonal development, neuroscience, neurotrophic signaling, visual system, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Axons/physiology*
  • Biological Transport/physiology
  • Blotting, Western
  • Cell Polarity/physiology*
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Primers/genetics
  • Genotype
  • Immunohistochemistry
  • In Situ Hybridization
  • Kinesins/genetics
  • Kinesins/metabolism*
  • Microscopy, Confocal
  • Microscopy, Electron, Transmission
  • Mitochondria/metabolism
  • Neurogenesis/physiology*
  • Neurotrophin 3/metabolism*
  • Real-Time Polymerase Chain Reaction
  • Signal Transduction/physiology*
  • Time-Lapse Imaging
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism*
PubMed
26076409 Full text @ Elife
Abstract
Development and function of highly polarized cells such as neurons depend on microtubule-associated intracellular transport, but little is known about contributions of specific molecular motors to the establishment of synaptic connections. In this study, we investigated the function of the Kinesin I heavy chain Kif5aa during retinotectal circuit formation in zebrafish. Targeted disruption of Kif5aa does not affect retinal ganglion cell differentiation, and retinal axons reach their topographically correct targets in the tectum, albeit with a delay. In vivo dynamic imaging showed that anterograde transport of mitochondria is impaired, as is synaptic transmission. Strikingly, disruption of presynaptic activity elicits upregulation of Neurotrophin-3 (Ntf3) in postsynaptic tectal cells. This in turn promotes exuberant branching of retinal axons by signaling through the TrkC receptor (Ntrk3). Thus, our study has uncovered an activity-dependent, retrograde signaling pathway that homeostatically controls axonal branching.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes