ZFIN ID: ZDB-FIG-150826-6
Auer et al., 2015 - Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLIFE   4 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 2 Outgrowth of the optic nerve and retinotopic mapping is normal in kif5aa mutants.

(A) Confocal imaging of the Tg(pou4f3:mGFP) transgene, labeling a subpopulation of Retinal Ganglion Cells (RGCs) with membrane bound GFP, at 48 hpf reveals that outgrowth of the optic nerve formed by RGC axons from the retina is not affected by the kif5aa mutation. Immunostaining against the Zn5 antigen (DM- GRASP/neurolin present within the visual system only on RGCs [Laessing and Stuermer, 1996; Fashena and Westerfield, 1999]) confirms that optic chiasm formation is normal (marked with an asterisk). No pathfinding errors occur at this level of axonal growth. Scale bars = 200 µm. Embryos facing upwards. R = rostral, C = caudal. (B) Injections of the lipophilic dyes DiI and DiO in different quadrants of the contralateral retina (depicted in the right panel) show that retinotopic mapping to the optic tectum is performed in the correct manner. Asterisks = pigment cells in the skin. D = dorsal, V = ventral, R = rostral, C = caudal. No misrouting of RGC axons to the ipsilateral tectum was observed (data not shown). Scale bars = 150 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama zn-5 WT standard conditions Long-pec cranial nerve II IHC
zn-5 Long-pec retinal ganglion cell axon IHC
zn-5 kif5aaic3023/ic3023 standard conditions Long-pec cranial nerve II IHC
zn-5 Long-pec retinal ganglion cell axon IHC
zn-5 kif5aaic3024/ic3024 standard conditions Long-pec cranial nerve II IHC
zn-5 Long-pec retinal ganglion cell axon IHC
GFP kif5aaic3023/ic3023; s356tTg standard conditions Long-pec cranial nerve II IFL
Long-pec retinal ganglion cell axon IFL
kif5aaic3024/ic3024; s356tTg standard conditions Long-pec cranial nerve II IFL
Long-pec retinal ganglion cell axon IFL
s356tTg standard conditions Long-pec cranial nerve II IFL
Long-pec retinal ganglion cell axon IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kif5aaic3023/ic3023 standard conditions Long-pec optic chiasma development process quality, normal
Long-pec optic nerve morphogenesis process quality, normal
Long-pec retinal ganglion cell axon guidance process quality, normal
kif5aaic3023/ic3023; s356tTg standard conditions Long-pec optic chiasma development process quality, normal
Long-pec optic nerve morphogenesis process quality, normal
Long-pec retinal ganglion cell axon guidance process quality, normal
kif5aaic3024/ic3024 standard conditions Long-pec optic chiasma development process quality, normal
Long-pec optic nerve morphogenesis process quality, normal
Long-pec retinal ganglion cell axon guidance process quality, normal
kif5aaic3024/ic3024; s356tTg standard conditions Long-pec optic chiasma development process quality, normal
Long-pec optic nerve morphogenesis process quality, normal
Long-pec retinal ganglion cell axon guidance process quality, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife