ZFIN ID: ZDB-FIG-150327-1
Glenn et al., 2014 - Distinct regulation of the anterior and posterior myeloperoxidase expression by Etv2 and Gata1 during primitive Granulopoiesis in zebrafish. Developmental Biology   393(1):149-59 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-15

Fig. 1

Etv2 function is necessary for neutropoiesis in the ALPM and is sufficient to initiate mpx expression. (A–K) Anterior but not posterior mpx expression is greatly reduced or absent in etv2y11mutants, as analyzed by in situ hybridization. (A and B) mpx expression is slightly reduced in the ICM region of etv2y11 mutants (B) as compared to their wild-type siblings (A) at the 20 somite stage. (C) Number of mpx-expressing cells at various stages in the anterior and posterior lateral plate mesoderm of wild type and etv2y11 mutant embryos. The cells were counted in 30 wt and 23 etv2y11 mutant embryos at the 20-somite stage, 16 wt and 16 mutant embryos at 24 hpf and 10 wt and 9 mutant embryos at 30 hpf. Error bars correspond to±standard deviation. Asterisks denote significant differences (p<0.05) calculated by Studentós T-Test. (D and E) mpx expression is strongly reduced at 24 hpf in the ALPM of etv2y11 mutants (E) compared to wild type siblings (D). (F and G) mpx expression has only a minor reduction in the ICM region of etv2y11 mutants (G) compared to their wild-type siblings (F). (H–K) mpx expression is reduced at 30 hpf in the anterior (I) and posterior (K) of etv2y11 mutants compared to wild type siblings (H and J). (L and M) gata1 expression is not significantly affected in etv2y11 mutants (M) as compared to wild-type siblings at 24 hpf (L). (N and O) cebpa expression at 24 hpf is strongly reduced in 100% of etv2y11 mutants (O, n=11) while it is strongly expressed in 100% of wild-type siblings (N, n=11). (P, Q) lyz expression is strongly reduced in 100% of etv2y11 mutants (Q, n=14) compared to 100% of wild-type siblings with normal lyz expression (P, n=15). (R–U) Etv2 DNA overexpression results in ectopic mpx expression as analyzed by in situ hybridization (R and S) and mpx:GFP live fluorescence (T and U) at the 20-somite stage (65% of etv2-injected embryos with increased or ectopic mpx:GFP, n=115 as opposed to 0% controls, n=39). Note that mpx:GFP exhibits non-specific GFP fluorescence in the neural tube. All embryos are positioned with the anterior to the left and dorsal side up.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cebpa WT standard conditions Prim-5 common myeloid progenitor ISH
etv2y11/y11; y1Tg standard conditions Prim-5 common myeloid progenitor ISH
gata1a WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
etv2y11/y11; y1Tg standard conditions Prim-5 intermediate cell mass of mesoderm ISH
GFP etv2y11/y11; uwm1Tg standard conditions 20-25 somites anatomical structure IFL
uwm1Tg standard conditions 20-25 somites anatomical structure IFL
lyz WT standard conditions Prim-5 neutrophil ISH
etv2y11/y11; y1Tg standard conditions Prim-5 neutrophil ISH
mpx WT standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
Prim-5 anterior lateral plate mesoderm ISH
Prim-5 intermediate cell mass of mesoderm ISH
Prim-15 intermediate cell mass of mesoderm ISH
Prim-15 rostral blood island ISH
etv2y11/y11; y1Tg standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
Prim-5 Not Detected anterior lateral plate mesoderm ISH
Prim-5 intermediate cell mass of mesoderm ISH
Prim-15 intermediate cell mass of mesoderm ISH
Prim-15 rostral blood island ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 393(1), Glenn, N.O., Schumacher, J.A., Kim, H.J., Zhao, E.J., Skerniskyte, J., Sumanas, S., Distinct regulation of the anterior and posterior myeloperoxidase expression by Etv2 and Gata1 during primitive Granulopoiesis in zebrafish, 149-59, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.