FIGURE

Fig. 2

ID

ZDB-FIG-140523-43

Publication

Swift et al., 2014 - SoxF factors and Notch regulate nr2f2 gene expression during venous differentiation in zebrafish

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Fig. 2

nr2f2 function is required for proper venous gene expression in the zebrafish vasculature. (A–D) Transmitted light (A and B) and green fluorescent light (C and D) images of control (A and C) and nr2f2 morpholino injected (B and D) Tg(fli1a-EGFP)^y1 transgenic animals. (E–L) Whole mount in situ hybridization of 24 hpf control (top) and nr2f2 morpholino injected (bottom) zebrafish embryo trunks probed for ephb4a (E), stab1 (F), lyve1 (G), sox7 (H), efnb2a (I), grl (J), tbx20 (K), and sox18 (L). Blue arrowheads note posterior cardinal vein gene expression, while red arrows note dorsal aorta gene expression. The inset numbers in panels F–L show the number of in situ-stained nr2f2 MO-injected embryos exhibiting the phenotype shown in the image panel over the total number of embryos examined. (M) Quantitative RT-PCR measurement of gene expression in excised trunks (see Materials and Methods) of 24 hpf control and nr2f2 morpholino injected animals. Values are all normalized to control gene expression levels, which are set equal to 1. Rostral is to the left and dorsal is up in all image panels. Scale bars, (A and B) 500µm, (C–L) 100µm.

Expression Data

Genes:	dab2 efnb2a EGFP ephb4a hey2 lyve1b prox1a sox7 sox18 stab1 tbx20
Fish:	EKW EKW + MO1-nr2f2 y1Tg y1Tg + MO1-nr2f2
Knockdown Reagent:	MO1-nr2f2
Anatomical Terms:	dorsal aorta posterior cardinal vein trunk vasculature blood vessel endothelium whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO1-nr2f2
Knockdown Reagent:	MO1-nr2f2
Observed In:	posterior cardinal vein venous blood vessel development
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 390, Swift, M.R., Pham, V.N., Castranova, D., Bell, K., Poole, R.J., Weinstein, B.M., SoxF factors and Notch regulate nr2f2 gene expression during venous differentiation in zebrafish, 116-25, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.