FIGURE

Fig. 5

ID
ZDB-FIG-140515-40
Publication
Sun et al., 2014 - Extraembryonic signals under the control of MGA, Max, and Smad4 are required for dorsoventral patterning
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Smad4 and Max Are Required in the YSL for BMP Signaling in the Embryo

Embryos injected into the YSL with 8 ng of maxmisMO (A–E), maxMO (F–J), smad4misMO>YSL (K–O), or smad4MO>YSL (P–T).

(A, A2, F, F2, K, K2, P, and P2) Image of a live morphants at prim-5.

(B, G, L, and Q) egr2b expression at prim-5.

(C, H, M, and R) gata1 expression at 8-somites (12 hpf).

(D, I, N, and S) pSmad1/5 gradient at 75% epiboly.

(E, J, O, and T) bmp2b/swr expression at 75% epiboly.

(U) The presence (blue) or absence (red) of the ventral tailfin was scored in smad4MO>YSL morphants injected with 100 pg β-galactosidase mRNA, 100 pg HA-smad4 mRNA, or 25 pg bmp2b/swr mRNA. Tailfin defects (red) were also scored in maxMO>YSL morphants injected with 100 pg β-galactosidase mRNA, 100 pg HA-max mRNA, or 25 pg bmp2b/swr mRNA. Anterior is to the left in (A)–(C), (F)–(H), (K)–(M), and (P)–(R). Dorsal is to the right in (D), (E), (I), (J), (N), (O), (S), and (T). p d 0.05.

See also Figures S2 and S5.

Expression Data
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 75%-epiboly to 5-9 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 28(3), Sun, Y., Tseng, W.C., Fan, X., Ball, R., and Dougan, S.T., Extraembryonic signals under the control of MGA, Max, and Smad4 are required for dorsoventral patterning, 322-334, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell